Identification of human sperm proteins involved in the interaction with homologous zona pellucida. 2003

Andrea Lasserre, and Fernanda González-Echeverría, and Carolina Moules, and Jorge G Tezón, and Patricia V Miranda, and Mónica H Vazquez-Levin
Instituto de Biología y Medicina Experimental, CONICET-UBA, Buenos Aires, Argentina.

OBJECTIVE To identify human sperm proteins involved in homologous zona pellucida (ZP) interaction. METHODS Prospective study. METHODS Basic research laboratory. METHODS Semen samples from normozoospermic donors, tissue sections from surgical pieces, and ZP from nonfertilized oocytes. METHODS Antibodies for sperm proteins (HSE; high salt extract) were developed (anti-HSE) and partially characterized. Participation of sperm proteins on ZP-interaction was tested with the hemizona assay (HZA). Antigens were immunolocalized in sperm and tissues. METHODS Sperm and tissue immunostaining; Western blotting; and number of sperm bound to the ZP. RESULTS Anti-HSE antibodies recognized several polypeptides in HSE (9 to 200 kd). Specific antibodies for 49 and 66 kd proteins (p49, p66) were obtained. Both (anti-p49 and anti-p66) stained the head of ejaculated and capacitated sperm. In the HZA, sperm preincubation with a mixture of anti-p49 and anti-p66 (100 micro g/mL) resulted in a decrease in the number of spermatozoa bound to the ZP. Presence of p66 (10 micro g/mL) inhibited sperm-ZP interaction. In contrast, p49 did not alter sperm binding to the ZP. Immunohistochemical analysis showed that p66 is present in the epididymis. No staining was observed in testicular sections. CONCLUSIONS We found that p66 is an epididymal protein that participates in human sperm interaction with homologous ZP.

UI MeSH Term Description Entries
D008297 Male Males
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013084 Sperm-Ovum Interactions Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION. Ovum-Sperm Interactions,Sperm Penetration,Egg-Sperm Interactions,Gamete Interactions,Oocyte-Sperm Interactions,Sperm-Egg Interactions,Sperm-Egg Penetration,Sperm-Oocyte Interactions,Sperm-Oocyte Penetration,Sperm-Ovum Penetration,Sperm-Zona Pellucida Penetration,Egg Sperm Interactions,Egg-Sperm Interaction,Gamete Interaction,Oocyte Sperm Interactions,Oocyte-Sperm Interaction,Ovum Sperm Interactions,Ovum-Sperm Interaction,Sperm Egg Interactions,Sperm Egg Penetration,Sperm Oocyte Interactions,Sperm Oocyte Penetration,Sperm Ovum Interactions,Sperm Ovum Penetration,Sperm Penetrations,Sperm Zona Pellucida Penetration,Sperm-Egg Interaction,Sperm-Egg Penetrations,Sperm-Oocyte Interaction,Sperm-Oocyte Penetrations,Sperm-Ovum Interaction,Sperm-Ovum Penetrations,Sperm-Zona Pellucida Penetrations
D013094 Spermatozoa Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility. Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D015044 Zona Pellucida A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings

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