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In an effort to better understand features in nucleotide analogs that result in the inhibition of HIV-1 reverse transcriptase, we have evaluated this enzyme with the 5'-triphosphate of the carbocyclic analog of 2'-deoxyguanosine (CdG-TP). CdG-TP was a reasonably potent competitive inhibitor of the incorporation of dGTP into DNA by HIV-1 reverse transcriptase using either a RNA or DNA template (Ki, 1 microM). CdG-TP was a good substrate for HIV-1 reverse transcriptase on both templates, but the DNA chain was poorly extended beyond the incorporation of CdG. These results indicate that substitution of ribose with a cyclopentane ring in nucleotides is not well tolerated by HIV-1 reverse transcriptase.
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
January 2007,
Antiviral chemistry & chemotherapy,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
April 1994,
Biochemical and biophysical research communications,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
February 1992,
The Journal of biological chemistry,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
March 1998,
Bioorganic & medicinal chemistry letters,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
October 1999,
Mutation research,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
November 1993,
Biochemistry,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
September 2006,
The Journal of biological chemistry,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
November 1992,
Science (New York, N.Y.),
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
October 1999,
Biochemistry,
W B Parker, and
E L White, and
S C Shaddix, and
L J Ross, and
W M Shannon, and
J A Secrist
October 1998,
The Journal of biological chemistry,
