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1. In the present study, we have investigated the distribution of nitric oxide synthase in the ferret brain. Nitric oxide synthase was determined biochemically and immunochemically. 2. In the rat brain, the highest nitric oxide synthase activity has been detected in the cerebellum. However, in the ferret brain, the highest activity was found in the striatum and the lowest in the cerebellum and cerebral cortex. The enzymatic activity was localized predominantly in the cytosolic fractions, it was dependent on NADPH and Ca2+, and inhibited by NG-nitro-L-arginine or NG-methyl-L-arginine. 3. Western blot analysis revealed that all regions of the ferret brain contained a 160 kD protein crossreacting with an antibody to nitric oxide synthase purified from the rat cerebellum, and the levels of relative intensity of staining by the antibody correlated with the distribution of nitric oxide synthase activity. 4. These results indicate that the ferret brain contains a nitric oxide synthase similar to the rat brain, but the distribution of enzymatic activity in the ferret brain differs markedly from the rat brain.
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
April 1994,
Journal of neurochemistry,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
January 1997,
Histology and histopathology,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
June 2003,
The Journal of comparative neurology,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
March 1995,
Urology,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
July 1994,
Philosophical transactions of the Royal Society of London. Series B, Biological sciences,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
February 1998,
Neuroscience letters,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
March 2002,
Neuroscience letters,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
January 1996,
Methods in enzymology,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
November 1993,
The American journal of physiology,
T Matsumoto, and
J A Mitchell, and
H H Schmidt, and
K L Kohlhaas, and
T D Warner, and
U Förstermann, and
F Murad
November 1998,
Journal of neurocytology,
