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A low molecular weight protein was obtained from a sonicate of armadillo-derived Mycobacterium leprae cells and from a lambda gt11 phage lysate of Escherichia coli (specifying the M. leprae 12-kDa protein) by a single step of ultrafiltration. Both proteins had an approximate molecular weight of about 12,000 (by SDS-PAGE) and were recognized by the M. leprae 12-kDa-specific monoclonal antibody ML06 by immunoblotting. Sera from 79 leprosy patients across the clinical spectrum, 17 contacts, and 12 normal healthy individuals were screened in an enzyme-linked immunosorbent assay (ELISA) using the 12-kDa proteins as the antigens. Antibodies to the 12-kDa protein (from lysate as well as sonicate) were detected in patients' sera across the clinical spectrum (44%-100% positivity), while no detectable reactivity was observed with control or contact sera. Sera from patients who had undergone a year or more of chemotherapy exhibited no reactivity compared to those from patients with only 3-6 months of chemotherapy. The 12-kDa proteins were also recognized by rabbit hyper-immune M. leprae antiserum.
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
December 2008,
Clinical and vaccine immunology : CVI,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
June 1990,
Leprosy review,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
May 1993,
Journal of clinical microbiology,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
September 1990,
Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ),
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
March 1988,
Clinical and experimental immunology,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
November 1990,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
July 1994,
Biological & pharmaceutical bulletin,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
October 1982,
Arerugi = [Allergy],
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
January 2018,
The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale,
R G Navalkar, and
R G Deshpande, and
M B Khan, and
L S Savariar, and
S R Navalkar
June 1993,
International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association,
