BIOMAT Database Logo BIOMAT Database Logo

Absence of one component of spectrin adenosine triphosphatase in hereditary spherocytosis. 1975

F H Kirkpatrick, and G M Woods, and P L La Celle

The stimulation by calcium and magnesium of ATPase activity of isolated ghosts, of water-soluble protein (spectrin), and of residual vesicles, derived from normal erythrocytes and from hereditary spherocytes (H.S.), has been measured. The ATPase activity found in normal water-soluble protein (WSP) at low levels of calcium (0.1-2.0 mM) is essentially absent in H.S. water-soluble protein, but the ATPase activity with magnesium and with high levels of calcium (60-100 mM) is the same in H.S. and normal WSP. Compared to normal, H.S. ghosts have increased Mg2+-stimulated activity. This increased activity is retained by the sedimentable vesicles ("residue") after extraction of the ghosts with 0.025 mM EDTA. The Ca2+, Mg2+-ATPase associated with the calcium pump is not significantly different in H.S.

UI MeSH Term Description Entries
D008274 Magnesium A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
D001798 Blood Proteins Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins. Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002413 Cations, Divalent Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis. Divalent Cations
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000251 Adenosine Triphosphatases A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA. ATPases,Adenosinetriphosphatase,ATPase,ATPase, DNA-Dependent,Adenosine Triphosphatase,DNA-Dependent ATPase,DNA-Dependent Adenosinetriphosphatases,ATPase, DNA Dependent,Adenosinetriphosphatases, DNA-Dependent,DNA Dependent ATPase,DNA Dependent Adenosinetriphosphatases,Triphosphatase, Adenosine
D013103 Spherocytosis, Hereditary A group of familial congenital hemolytic anemias characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. The erythrocytes have increased osmotic fragility and are abnormally permeable to sodium ions. Hereditary Spherocytoses,Spherocytoses, Hereditary

Related Publications

F H Kirkpatrick, and G M Woods, and P L La Celle
Spectrin mutations in hereditary elliptocytosis and hereditary spherocytosis.
January 1996, Human mutation,
F H Kirkpatrick, and G M Woods, and P L La Celle
Partial deficiency of erythrocyte spectrin in hereditary spherocytosis.
January 1985, Nature,
F H Kirkpatrick, and G M Woods, and P L La Celle
Isoelectric focusing of spectrin components in hereditary spherocytosis.
September 1976, Clinica chimica acta; international journal of clinical chemistry,
F H Kirkpatrick, and G M Woods, and P L La Celle
Combined ankyrin and spectrin deficiency in hereditary spherocytosis.
August 1993, Annals of hematology,
F H Kirkpatrick, and G M Woods, and P L La Celle
The abnormal phosphorylation of spectrin in human hereditary spherocytosis.
November 1981, Biochimica et biophysica acta,
F H Kirkpatrick, and G M Woods, and P L La Celle
ADENOSINE TRIPHOSPHATE METABOLISM IN HEREDITARY SPHEROCYTOSIS.
August 1965, The Journal of clinical investigation,
F H Kirkpatrick, and G M Woods, and P L La Celle
Relative deficiency of Ca2 plus-dependent adenosine triphosphatase activity of red cell membranes in hereditary spherocytosis.
May 1974, Biochemical and biophysical research communications,
F H Kirkpatrick, and G M Woods, and P L La Celle
Studies on calcium transport and calcium-dependent adenosine triphosphatase activity of erythrocyte membranes in hereditary spherocytosis.
December 1976, British journal of haematology,
F H Kirkpatrick, and G M Woods, and P L La Celle
Hereditary spherocytosis with spectrin deficiency due to an unstable truncated beta spectrin.
March 1996, Blood,
F H Kirkpatrick, and G M Woods, and P L La Celle
Hereditary spherocytosis characterized by increased spectrin/band 3 ratio.
January 1992, British journal of haematology,
Copied contents to your clipboard!