BIOMAT Database Logo BIOMAT Database Logo

Immunohistochemical detection of Pneumocystis carinii in transbronchial lung biopsy specimens: antigen difference between human and rat Pneumocystis carinii. 1992

M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Third Department of Internal Medicine, Kochi Medical School, Nankoku.

Transbronchial lung biopsy specimens, from three patients with non-AIDS-related Pneumocystis carinii pneumonia (PCP) and rat lung tissue in which PCP was induced by the administration of dexamethasone, were studied to determine the diagnostic usefulness of an immunohistochemical method using commercially available anti-Pneumocystis monoclonal antibody, 3F6, on formalin-fixed, paraffin-embedded tissue. PC was consistently stained a bright red color and unambiguously identified in all three human lung specimens, but not stained in lung tissues at autopsy from patients with various fungal pneumonias. In contrast, PC was weakly stained in PCP-induced rat lungs. The present study indicates human PC and rat PC to be antigenically different in terms of the existence of the 82 kilo-dalton (kD) antigen against which 3F6 is directed.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008297 Male Males
D008709 Methenamine An anti-infective agent most commonly used in the treatment of urinary tract infections. Its anti-infective action derives from the slow release of formaldehyde by hydrolysis at acidic pH. (From Martindale, The Extra Pharmacopoeia, 30th ed, p173) Hexamethylenetetramine,Hexamine,Aminoform,Hexamine Silver,Methenamine Silver,Silver Methenamine,Urotropin,Methenamine, Silver,Silver, Hexamine,Silver, Methenamine
D011010 Pneumocystis A genus of ascomycetous FUNGI, family Pneumocystidaceae, order Pneumocystidales. It includes various host-specific species causing PNEUMOCYSTIS PNEUMONIA in humans and other MAMMALS. Pneumocysti
D011020 Pneumonia, Pneumocystis A pulmonary disease in humans occurring in immunodeficient or malnourished patients or infants, characterized by DYSPNEA, tachypnea, and HYPOXEMIA. Pneumocystis pneumonia is a frequently seen opportunistic infection in AIDS. It is caused by the fungus PNEUMOCYSTIS JIROVECII. The disease is also found in other MAMMALS where it is caused by related species of Pneumocystis. P carinii Pneumonia,P. carinii Pneumonia,P. jirovecii Pneumonia,PCP Pneumonia,Pneumocystis Pneumonia,Pneumocystosis,Pneumonia, Interstitial Plasma Cell,PCP Infection,Pneumocystis carinii Pneumonia,Pneumocystis jirovecii Pneumonia,Pneumonia, Pneumocystis carinii,Infection, PCP,P carinii Pneumonias,P. carinii Pneumonias,P. jirovecii Pneumonias,PCP Infections,PCP Pneumonias,Pneumocystis Pneumonias,Pneumocystoses,Pneumonia, P carinii,Pneumonia, P. carinii,Pneumonia, P. jirovecii,Pneumonia, PCP,Pneumonia, Pneumocystis jirovecii,Pneumonias, PCP
D011650 Pulmonary Alveoli Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place. Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults

Related Publications

M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Calcofluor white stain for the detection of Pneumocystis carinii in transbronchial lung biopsy specimens: a study of 68 cases.
August 1996, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Pneumocystis carinii antigen detection in rat serum and lung lavage.
September 1988, Journal of clinical microbiology,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
[Transbronchial lung biopsy in Pneumocystis carinii pneumonia following therapy of adult leukemia].
September 1982, Nihon Kyobu Shikkan Gakkai zasshi,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Transbronchial forceps lung biopsy through the fiberoptic bronchoscope in Pneumocystis carinii pneumonia.
September 1974, Chest,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Comparison of processing techniques for detection of Pneumocystis carinii cysts in open-lung biopsy specimens.
February 1985, Journal of clinical microbiology,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
[EGFR gene detection using transbronchial lung biopsy specimens].
February 2011, Zhonghua bing li xue za zhi = Chinese journal of pathology,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Pneumocystis carinii pneumonia in human immunodeficiency virus infected patients in Bombay: diagnosed by bronchoalveolar lavage cytology and transbronchial lung biopsy.
January 1996, The Indian journal of chest diseases & allied sciences,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Pneumocystis carinii pneumonia. Diagnosis by lung biopsy.
June 1975, The American journal of medicine,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
Direct detection of Pneumocystis carinii in fresh bronchoalveolar lavage specimens.
May 1991, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
M Kobayashi, and T Moriki, and Y Uemura, and N Takehara, and I Kubonishi, and H Taguchi, and I Miyoshi
PNEUMOCYSTIS CARINII INFECTION DIAGNOSED BY ANTEMORTEM LUNG BIOPSY.
May 1965, The Annals of thoracic surgery,
Copied contents to your clipboard!