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To examine the pathogenetic role of platelet-derived growth factor (PDGF) in hypertensive kidney damage, we studied the gene expression of PDGF A-chain and B-chain in an animal model of malignant hypertension. Experimental malignant hypertension induced by unilateral nephrectomy combined with deoxycorticosterone and salt loading in the spontaneously hypertensive rat resulted in severely elevated blood pressure and renal histological damage, characterized by necrotizing vasculitis. Using reverse transcription-polymerase chain reaction analysis followed by Southern blot analysis, we observed that PDGF B-chain gene expression was increased in the kidney of experimental malignant hypertension and was correlated with the severity of glomerular damage, while PDGF A-chain gene expression was unaffected. Antihypertensive treatment with manidipine reduced glomerular damage and a decreased gene expression of PDGF B-chain. These results suggest that PDGF B-chain may have a role in mediating hypertensive kidney damage.
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
January 1995,
The Journal of bone and joint surgery. American volume,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
December 1989,
Kidney international,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
September 1984,
Biochemical pharmacology,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
November 1991,
Hypertension (Dallas, Tex. : 1979),
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
July 2008,
American journal of respiratory and critical care medicine,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
April 2008,
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
January 1984,
Nordisk medicin,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
February 1998,
Biochemical Society transactions,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
April 1994,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
M Okamura, and
Y Konishi, and
M Nishimura, and
N Negoro, and
N Umetani, and
T Inoue, and
Y Kanayama, and
T Takeda
September 2000,
Molecular and cellular biochemistry,
