BIOMAT Database Logo BIOMAT Database Logo

Trans-species transfer of Wolbachia: microinjection of Wolbachia from litomosoides sigmodontis into Acanthocheilonema viteae. 2003

N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Department of Molecular Parasitology, Institute of Biology, Humboldt University, Berlin, Germany.

Intracellular bacteria of the genus Wolbachia are found in most filarial nematodes, but are lacking in some species like Acanthocheilonema viteae. Due to their symbiotic nature and their role in the pathology of filarial infections they are considered to be potential targets for intervention against filarial infections in man. Infection of A. viteae (a species which does not naturally carry Wolbachia) with Wolbachia bacteria could allow comparative studies on the effect of the endobacterium on the parasite and on the host's immune systems. As a step towards such studies we microinjected adult female A. viteae with Wolbachia obtained from Litomosoides sigmodontis. The bacteria were isolated from L. sigmodontis by density-gradient centrifugation, microinjected into A. viteae worms and bacterial DNA detected by PCR with Wolbachia specific primers (ftsZ gene). Microinjected worms were cultured in vitro, and 81% survived for 10 days. Implantation of microinjected worms into Meriones unguiculatus, the rodent host of A. viteae resulted in 38% survival. The DNA of the microinjected worms recovered from jirds 8 weeks after implantation contained Wolbachia DNA as shown by PCR, suggesting that Wolbachia of L. sigmodontis can be horizontally transmitted to A. viteae.

UI MeSH Term Description Entries
D008845 Microinjections The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes. Microinjection
D004153 Dipetalonema A filarial nematode parasite of mammalian blood with the vector being a tick or small fly. Dipetalonemas
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005370 Filarioidea A superfamily of nematodes of the order SPIRURIDA. Members possess a filiform body and a mouth surrounded by papillae. Litomosoides,Filarioideas,Litomosoide
D005849 Gerbillinae A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys. Gerbils,Jird,Meriones,Psammomys,Rats, Sand,Gerbil,Jirds,Merione,Rat, Sand,Sand Rat,Sand Rats
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013559 Symbiosis The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other. Endosymbiosis,Commensalism,Mutualism
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D018509 DNA, Helminth Deoxyribonucleic acid that makes up the genetic material of helminths. Helminth DNA
D020577 Wolbachia A genus of bacteria which comprises a heterogenous group of gram-negative small rods and coccoid forms associated with arthropods. (From Bergey's Manual of Systematic Bacteriology, vol 1, 1984)

Related Publications

N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Human filariasis-contributions of the Litomosoides sigmodontis and Acanthocheilonema viteae animal model.
December 2021, Parasitology research,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Effects of Bay 44-4400, a new cyclodepsipeptide, on developing stages of filariae (Acanthocheilonema viteae, Brugia malayi, Litomosoides sigmodontis) in the rodent Mastomys coucha.
September 2001, Acta tropica,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Does Litomosoides sigmodontis synthesize dimethylethanolamine from choline?
January 2008, Parasitology,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Targeting of wolbachia endobacteria in litomosoides sigmodontis: comparison of tetracyclines with chloramphenicol, macrolides and ciprofloxacin
April 2000, Tropical medicine & international health : TM & IH,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Targeting of Wolbachia endobacteria in Litomosoides sigmodontis: comparison of tetracyclines with chloramphenicol, macrolides and ciprofloxacin.
April 2000, Tropical medicine & international health : TM & IH,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
In vitro effects of 2-tert-butyl-benzothiazole derivatives on microfilariae of Litomosoides carinii, Brugia malayi and Acanthocheilonema viteae.
July 1991, Arzneimittel-Forschung,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
In vivo efficacy of the boron-pleuromutilin AN11251 against Wolbachia of the rodent filarial nematode Litomosoides sigmodontis.
January 2020, PLoS neglected tropical diseases,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Acanthocheilonema viteae: transplacental transmission of microfilariae from mother to offspring.
January 1996, Applied parasitology,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Combinations of registered drugs reduce treatment times required to deplete Wolbachia in the Litomosoides sigmodontis mouse model.
January 2018, PLoS neglected tropical diseases,
N Hartmann, and H Stuckas, and R Lucius, and W Bleiss, and F Theuring, and B H Kalinna
Secreted microRNA data from the parasitic filarial nematode Acanthocheilonema viteae.
April 2020, Data in brief,
Copied contents to your clipboard!