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Synthesis and properties of phosphorylated 3'-O-beta-D-ribofuranosyl-2'-deoxythymidine. 2003
Ekaterina V Efimtseva, and
Alexandra A Shelkunova, and
Sergey N Mikhailov, and
Koen Nauwelaerts, and
Jef Rozenski, and
Eveline Lescrinier, and
Piet Herdewijn
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
A strategy was developed for the synthesis of 3'-O-beta-D-ribofuranosyl 2'-deoxythymidine derivatives using three different protecting groups, which allows the synthesis of a phosphoramidite building block for oligonucleotide synthesis. Likewise the 5'-O- and 5''-O-phosphorylated analogues were synthesized and their conformation was determined using NMR spectroscopy.
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