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Further investigation of the epitope recognized by the new monoclonal antibody 2C9. 2003
Keitaro Kato, and
Kazuhiro Yoshikawa, and
Tomohiro Taki, and
Kenya Shitara, and
Kazuyasu Nakamura, and
Maiko Hirota, and
Nobuo Hanai, and
Kogenta Nakamura, and
Hiroto Kokubo, and
Kenji Mitsui, and
Yoshiaki Yamada, and
Nobuaki Honda, and
Ryuzo Ueda, and
Shinsuke Saga, and
Hidetoshi Fukatsu
Department of Urology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.
OBJECTIVE We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody. METHODS The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system. RESULTS The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA. CONCLUSIONS The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.
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