Isolation and characterization of a Brassica napus cDNA corresponding to a B-class floral development gene. 2003

Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
Department of Biology, 112 Science Place, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E2, Canada.

B-class floral homeotic genes are required for the proper formation and identity of petals and stamens in dicot flowers. A partial cDNA clone encoding a B-class gene, BnAP3 (Brassica napus APETALA3), was isolated from a B. napus cDNA library derived from young inflorescence meristems. The 5' region of the cDNA was retrieved by RACE. The deduced amino acid sequence of the full-length clone exhibited high similarity to APETALA3 of Arabidopsis thaliana and functionally homologous proteins from other species. 5' RACE and Southern analysis suggests that BnAP3 has multiple alleles in B. napus. Expression analysis assayed by RT-PCR shows that BnAP3 is expressed in floral tissues, as well as non-floral tissues such as root and bract. Transformation of wild-type A. thaliana and B. napus plants with BnAP3 under the control of a promoter specific to reproductive organs converts carpels to stamens, while the expression of this construct in A. thaliana plants mutant for AP3 restores the development of third-whorl stamens in addition to directing a carpel to stamen conversion in the fourth whorl.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D017386 Sequence Homology, Amino Acid The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species. Homologous Sequences, Amino Acid,Amino Acid Sequence Homology,Homologs, Amino Acid Sequence,Homologs, Protein Sequence,Homology, Protein Sequence,Protein Sequence Homologs,Protein Sequence Homology,Sequence Homology, Protein,Homolog, Protein Sequence,Homologies, Protein Sequence,Protein Sequence Homolog,Protein Sequence Homologies,Sequence Homolog, Protein,Sequence Homologies, Protein,Sequence Homologs, Protein
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D018507 Gene Expression Regulation, Developmental Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism. Developmental Gene Expression Regulation,Embryologic Gene Expression Regulation,Gene Expression Regulation, Embryologic,Regulation of Gene Expression, Developmental,Regulation of Gene Expression, Embryologic,Regulation, Gene Expression, Developmental,Regulation, Gene Expression, Embryologic
D029688 Brassica napus A plant species of the family BRASSICACEAE best known for the edible roots. Rapeseed,Rutabaga,Turnip,Rapeseeds,Turnips

Related Publications

Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
November 1993, Plant molecular biology,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
December 1993, Plant molecular biology,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
January 2004, Cellular & molecular biology letters,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
November 1996, Plant molecular biology,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
May 1994, Plant physiology,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
April 1991, Plant molecular biology,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
April 1991, Genetical research,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
November 2006, Plant physiology and biochemistry : PPB,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
June 2003, Molecular plant-microbe interactions : MPMI,
Jeffrey D Pylatuik, and Donna L Lindsay, and Arthur R Davis, and Peta C Bonham-Smith
May 1994, Plant physiology,
Copied contents to your clipboard!