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The aim of the study was to work out a method for determining the soluble transferrin receptor (TfR) on the basis of the direct immune-enzyme analysis, including its dynamic testing in patients with anemia of various etiologies. Reagents, needed for the above analysis, i.e. TfRs and antibodies to them, were obtained to implement the set goal. TfR was isolated by Kanevsky's affine chromatography from the homogenate of patients. The thus isolated TfR was used to immunize and to obtain the monoclonal antibodies. The conjugate of horseradish peroxidase (HP) and antibodies to TfR were made use of to determine the quantity of bound antibodies. One type of antibodies were immobilized in plates of the "Nunk" company (Denmark), and the other type of monoclonal antibodies were conjugated with HP. A reliably higher TfR in iron-deficiency anemia was shown during the determination of the TfR level in 36 donors and 266 patients. After a conducted ferrotherapy, the TfR level went down approaching the normal value. The TfR level was related with a disease stage and activity of the prolipherative process in cases of autoimmune hemolytic anemia, B12-folate-dependent anemia, lympho- and myeloprolipherative diseases and in oncology patients. The elaborated method opens up new possibilities for the differential diagnosis of anemia and provide for objective criteria of a conducted therapy.
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
October 2002,
International journal of hematology,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
April 2000,
Clinical chemistry and laboratory medicine,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
May 1999,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
December 2010,
Critical reviews in clinical laboratory sciences,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
July 1995,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
January 1997,
Zhonghua nei ke za zhi,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
January 1996,
Acta medica (Hradec Kralove),
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
February 2004,
The Tohoku journal of experimental medicine,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
October 2007,
The Medical journal of Malaysia,
A A Levina, and
A E Kogan, and
T V Bulgarina, and
Iu I Mamukova, and
N V Tsvetaeva, and
M M Tsibul'skaia, and
T V Kaziukova
October 2000,
La Revue de medecine interne,
