Specific association of glycoprotein B with lipid rafts during herpes simplex virus entry. 2003

Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. fbender@biochem.dental.upen.edu

Herpes simplex virus (HSV) entry requires the interaction of glycoprotein D (gD) with a cellular receptor such as herpesvirus entry mediator (HVEM or HveA) or nectin-1 (HveC). However, the fusion mechanism is still not understood. Since cholesterol-enriched cell membrane lipid rafts are involved in the entry of other enveloped viruses such as human immunodeficiency virus and Ebola virus, we tested whether HSV entry proceeds similarly. Vero cells and cells expressing either HVEM or nectin-1 were treated with cholesterol-sequestering drugs such as methyl-beta-cyclodextrin or nystatin and then exposed to virus. In all cases, virus entry was inhibited in a dose-dependent manner, and the inhibitory effect was fully reversible by replenishment of cholesterol. To examine the association of HVEM and nectin-1 with lipid rafts, we analyzed whether they partitioned into nonionic detergent-insoluble glycolipid-enriched membranes (DIG). There was no constitutive association of either receptor with DIG. Binding of soluble gD or virus to cells did not result in association of nectin-1 with the raft-containing fractions. However, during infection, a fraction of gB but not gC, gD, or gH associated with DIG. Similarly, when cells were incubated with truncated soluble glycoproteins, soluble gB but not gC was found associated with DIG. Together, these data favor a model in which HSV uses gB to rapidly mobilize lipid rafts that may serve as a platform for entry and cell signaling. It also suggests that gB may interact with a cellular molecule associated with lipid rafts.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D011991 Receptors, Virus Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response. Viral Entry Receptor,Viral Entry Receptors,Virus Attachment Factor,Virus Attachment Factors,Virus Attachment Receptor,Virus Attachment Receptors,Virus Entry Receptor,Virus Entry Receptors,Virus Receptor,Virus Receptors,Attachment Factor, Virus,Attachment Factors, Virus,Attachment Receptor, Virus,Attachment Receptors, Virus,Entry Receptor, Viral,Entry Receptor, Virus,Entry Receptors, Viral,Entry Receptors, Virus,Receptor, Viral Entry,Receptor, Virus,Receptor, Virus Attachment,Receptor, Virus Entry,Receptors, Viral Entry,Receptors, Virus Attachment,Receptors, Virus Entry
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D002784 Cholesterol The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils. Epicholesterol
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000075983 Nectins A family of calcium-independent cell adhesion molecules of the immunoglobulin superfamily. They are expressed by most cell types and mediate both homotypic and heterotypic cell-cell adhesion. Nectins function in a variety of morphogenetic and developmental processes that include organogenesis of the eye, ear, tooth, and cerebral cortex; they also play roles in viral infection and cell proliferation. CD111 Antigen,CD112 Antigen,CD113 Antigen,Herpesvirus Entry Mediator C,HevC Protein,Human Poliovirus Receptor Related 1,Nectin,Nectin-1,Nectin-1delta,Nectin-2,Nectin-2alpha,Nectin-2delta,Nectin-2gamma,Nectin-3,Nectin1delta,PVRL1,Poliovirus Receptor-Related Protein 1,Prr1 Protein,Antigen, CD111,Antigen, CD112,Antigen, CD113,Nectin 1,Nectin 1delta,Nectin 2,Nectin 2alpha,Nectin 2delta,Nectin 2gamma,Nectin 3,Poliovirus Receptor Related Protein 1
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012995 Solubility The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Solubilities
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell

Related Publications

Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
March 2008, Cell,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
September 1996, Journal of virology,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
November 1986, Journal of virology,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
February 2020, mSphere,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
December 2007, Virus genes,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
October 1993, Journal of virology,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
February 1989, Journal of virology,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
September 2020, Science advances,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
August 1988, Journal of virology,
Florent C Bender, and J Charles Whitbeck, and Manuel Ponce de Leon, and Huan Lou, and Roselyn J Eisenberg, and Gary H Cohen
March 2012, Journal of virology,
Copied contents to your clipboard!