Biomaterial Database

[Physico-chemical and enzymatic properties of contractile protein of rat liver mitochondria]. 1975

A N Stozharov, and A N Razumovich

Some properties of the contractile protein of mitochondria in rat liver (mito-AM) and actomyosin (AM) have been comparatively studied. Mito-AM revealed the viscosity change as well as the precipitation test when ATP was added. The precipitation test rate proved to be higher in some cases than in case of AM. The characteristic feature for both enzymes was the inhibition of the enzymatic activity due to substrate abundance and the reaction product. According to their activating eff on ATP-ase of mito-AM, divalent cations can be placed in the following way: Ca2+ greater than greater than Mg2+greater than Mn2+greater than Zn2+. A similarity in the effect of p-chlormercuribenzoate, amitale and ouabain on mito-AM and AM is revealed. Spectrum analysis data (absorption and fluorescent spectra, fluorescence polarization) as well as different substrate specificity indicate the presence of certain differences in proteins studied. Purified mito-AM revealed a number of components with mollecular meights ranging from 12,500 to 87,000 under polyacrylamide gel electrophoresis in the presence of sodium dodecul sulphate. The 44,000 mollecular weight polypeptide chain was of muscle actin origin. The results obtained are discussed from the view point of protein functional characteristics.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008930	Mitochondria, Liver	Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)	Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002413	Cations, Divalent	Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.	Divalent Cations
D002729	Chloromercuribenzoates	Chloride and mercury-containing derivatives of benzoic acid.
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D000199	Actins	Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.	F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin
D000205	Actomyosin	A protein complex of actin and MYOSINS occurring in muscle. It is the essential contractile substance of muscle.