-transparent.png){kind=logo}
The kinetic of plasmin, Va1442-plasmin, Lys530-plasmin inhibition reaction by alpha 2-antiplasmin as well as interaction of the inhibitor with different derivatives of the plasminogen and its fragments were studied. It was shown that plasmin, mini- and micro-plasmin activity decreased by 97, 88 and 85%, respectively, for equimolar ratio 1:1 of the inhibitor. The value of the inhibition reached its maximum in 1-2, 5-10 and 10-15 min, respectively. The constants of the complex formation rate were 1.4 x 10(6); 1.7 x 10(5) and 6.2 x 10(4) M-1s-1 for the plasmin, mini- and micro-plasmin with alpha 2-antiplasmin, respectively. Both 10(-2) M 6-aminohexanoic acid and 10(-1) M arginine reduced the complex formation rate between plasmin, mini-plasmin and alpha 2-antiplasmin to the value of the rate reaction between micro-plasmin and inhibitor. alpha 2-Antiplasmin bound with all investigated derivatives and fragments of plasminogen. The amount of inhibitor decreased in the series: plasmin, kringle 1-3, kringle 4, mini-plasminogen, micro-plasminogen. The kringle 1-4 and kringle 5 were determined to control the rate of reaction between enzyme and inhibitor, being not necessary for the inhibition. The comparison of the inhibitor interaction with DPP-plasmin, mini-plasminogen and micro-plasminogen displayed the possibility of the additional region existence in catalytic domain. This region participated in the complex with alpha 2-antiplasmin formation. It is supposed that the multisite interaction between plasmin and alpha 2-antiplasmin provides for the specificity and efficiency the inhibitor action.
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
October 2000,
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
July 1979,
Biochimica et biophysica acta,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
June 1999,
Thrombosis and haemostasis,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
May 1996,
FEBS letters,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
May 1993,
The Journal of biological chemistry,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
November 1996,
American journal of hematology,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
October 1946,
Lancet (London, England),
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
July 2002,
Thrombosis and haemostasis,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
July 2008,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
T V Hrynenko, and
O I Iusova, and
M B Zadorozhna, and
Ie M Makohonenko
May 1978,
Biochimica et biophysica acta,
