Efficient thymic immigration of B220+ lymphoid-restricted bone marrow cells with T precursor potential. 2003

Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
Department of Pathology, Harvard Medical School, Dana-Farber Cancer Institute Boston, Massachusetts, 02115, USA.

Using a human CD25 reporter transgene controlled by regulatory sequences from the gene encoding pre-T cell receptor alpha, we identified a common lymphocyte precursor (CLP-2) population that, in contrast to the previously identified CLP-1 population, was c-Kit-B220+. In short-term culture, the CLP-2 could be derived from the CLP-1 subset, and contained cells that in clonogenic assays were assessed to be bipotent precursors of T and B cells. Intravenous injection of bone marrow cells yielded a selective accumulation of CLP-2 thymic immigrants that in thymic organ culture generated mature alphabeta T cells. Although the CLP-2 subset may represent the most differentiated population with T cell potential before commitment to the B cell lineage, other subsets of thymic immigrants capable of generating T cells may exist.

UI MeSH Term Description Entries
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001402 B-Lymphocytes Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. B-Cells, Lymphocyte,B-Lymphocyte,Bursa-Dependent Lymphocytes,B Cells, Lymphocyte,B Lymphocyte,B Lymphocytes,B-Cell, Lymphocyte,Bursa Dependent Lymphocytes,Bursa-Dependent Lymphocyte,Lymphocyte B-Cell,Lymphocyte B-Cells,Lymphocyte, Bursa-Dependent,Lymphocytes, Bursa-Dependent
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated

Related Publications

Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
September 1974, Cellular immunology,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
September 1985, Proceedings of the National Academy of Sciences of the United States of America,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
July 1996, Cellular immunology,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
July 2010, Blood,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
May 1990, Experimental hematology,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
September 2006, Journal of immunology (Baltimore, Md. : 1950),
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
February 1981, Journal of immunology (Baltimore, Md. : 1950),
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
January 1997, Journal of immunology (Baltimore, Md. : 1950),
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
January 2000, Developmental immunology,
Colin H Martin, and Iannis Aifantis, and M Lucila Scimone, and Ulrich H von Andrian, and Boris Reizis, and Harald von Boehmer, and Fotini Gounari
October 1973, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!