ABO and Rh phenotyping by absorption-elution technique using cerebral dura maters. 2003

Toshimitsu Watabiki, and Atsushi Akane, and Yutaka Okii, and Sumitaka Yoshimura, and Takuma Tokiyasu, and Manabu Yoshida
Department of Legal Medicine, Kansai Medical University, Moriguchi 570-8506, Japan. watabiki@takii.kmu.ac.jp

Phenotyping of ABO and Rh blood groups was performed by the absorption-elution technique using cerebral dura maters. For the ABO system, the cerebral dura maters extracted from nine autopsied cadavers including two burnt bodies, two putrefied corpses, and one half-mummified and one skeletal structure were tested with commercially available Wako antisera (animal polyclonal antibodies). At the same time, blood, fingernails or bones were sampled. In all the cases, the phenotypes could be typed correctly and more clearly with the use of 2 x 2 x 0.3 mm dura maters (0.6 mm thick dura maters were sliced to 0.3 mm thickness) than the phenotyping using 2 x 2 mm fingernails or 2 x 2 x 1 mm bones. For the Rh system, the cerebral dura maters extracted from eight autopsied cadavers within 2 days after death including two burnt bodies were tested with commercially available Ortho Bioclone anti-C, anti-c, anti-E and anti-e sera (human monoclonal antibodies), and Ortho anti-D serum (human polyclonal antibody). The eluate of anti-D antibody was needed to perform the indirect anti-globulin test (Ortho Coombs serum). At the same time, blood was sampled. In all the cases, the Rh blood groups of cerebral dura maters were in agreement with those of blood.

UI MeSH Term Description Entries
D007106 Immune Sera Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen. Antisera,Immune Serums,Sera, Immune,Serums, Immune
D009262 Nails The thin, horny plates that cover the dorsal surfaces of the distal phalanges of the fingers and toes of primates. Fingernails,Toenails,Fingernail,Nail,Toenail
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D011884 Radius The outer shorter of the two bones of the FOREARM, lying parallel to the ULNA and partially revolving around it. Radial Tuberosity,Radial Tuberosities,Tuberosities, Radial,Tuberosity, Radial
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D004388 Dura Mater The outermost of the three MENINGES, a fibrous membrane of connective tissue that covers the brain and the spinal cord. Falx Cerebelli,Falx Cerebri,Pachymeninx,Tentorium Cerebelli
D005554 Forensic Medicine The application of medical knowledge to questions of law. Legal Medicine,Medicine, Forensic,Medicine, Legal
D006386 Hemagglutination Tests Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed) Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000017 ABO Blood-Group System The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane. ABH Blood Group,ABO Blood Group,ABO Factors,Blood Group H Type 1 Antigen,H Blood Group,H Blood Group System,ABO Blood Group System,Blood Group, ABH,Blood Group, ABO,Blood Group, H,Blood-Group System, ABO,Factors, ABO,System, ABO Blood-Group

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