Electrophoretic analysis of ITS from Piscirickettsia salmonis Chilean isolates. 2003

Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Alameda 3363, Casilla 40 Correo 33, Santiago, Chile.

Piscirickettsia salmonis is the most important pathogen in salmonid mariculture in Chile. Since it was reported numerous piscirickettsiosis outbreaks have occurred differing in virulence and mortality. Genetic variability of P. salmonis isolates has been suggested as one factor to explain this. However until now isolates obtained from outbreaks have not been analyzed. Knowledge of genetic variability of P. salmonis is very limited and also a useful screening method for genetic variations in isolates without sequencing is not available. Here we report an electrophoretic analysis of internal transcribed spacer region (ITS) of eleven P. salmonis isolates obtained from different salmon species and places in southern Chile. When PCR products were submitted to polyacrylamide gel electrophoresis (PAGE) a characteristic electrophoretic pattern was observed, distinguishable from ITS of other bacteria, including fish pathogens. Even though this pattern is conserved in all isolates, a difference in ITS electrophoretic mobility was observed, determining clearly two groups: ITS with higher or with lower electrophoretic mobility, including LF-89 and EM-90 isolates, respectively. A higher ITS sequence homology inside each group was shown by heteroduplex mobility assay (HMA). Our results show that genetic variability between Chilean P. salmonis isolates allows the differentiation of two groups with similar behavior observed previously when six P. salmonis isolates from three geographic origins were analyzed by 16S, 23S and ITS sequencing. PAGE analysis of ITS and HMA could be a basis to develop an assay for screening genetic variability between P. salmonis isolates.

UI MeSH Term Description Entries
D009692 Nucleic Acid Heteroduplexes Double-stranded nucleic acid molecules (DNA-DNA or DNA-RNA) which contain regions of nucleotide mismatches (non-complementary). In vivo, these heteroduplexes can result from mutation or genetic recombination; in vitro, they are formed by nucleic acid hybridization. Electron microscopic analysis of the resulting heteroduplexes facilitates the mapping of regions of base sequence homology of nucleic acids. Heteroduplexes, Nucleic Acid,Heteroduplex DNA,Acid Heteroduplexes, Nucleic,DNA, Heteroduplex
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002677 Chile A country in southern South America, bordering the South Pacific Ocean, between Argentina and Peru.
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012487 Salmonidae A family of anadromous fish comprising SALMON; TROUT; whitefish; and graylings. They are the most important food and game fishes. Their habitat is the northern Atlantic and Pacific, both marine and inland, and the Great Lakes. (Nelson: Fishes of the World, 1976, p97) Graylings,Thymallus,Whitefish,Salmonids,Grayling,Salmonid
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017684 Oncorhynchus kisutch An anadromous species of SALMON ranging from the Arctic and Pacific Oceans to Monterey Bay, California and inhabiting ocean and coastal streams. It is familiarly known as the coho or silver salmon. It is relatively small but its light-colored flesh is of good flavor. Salmon, Coho,Salmon, Silver,Coho Salmon,Silver Salmon

Related Publications

Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
April 2016, Journal of fish diseases,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
April 2015, Journal of fish diseases,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
March 2016, BMC veterinary research,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
January 2016, PloS one,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
August 2017, Journal of fish diseases,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
July 2004, Applied and environmental microbiology,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
December 2019, Journal of fish diseases,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
October 2017, Journal of fish diseases,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
November 2016, Veterinary microbiology,
Andrés Casanova, and Johanna R Obreque C, and Aldo Gaggero, and Erik Landskron, and Ana M Sandino G, and Matilde Jashés M
January 1999, Diseases of aquatic organisms,
Copied contents to your clipboard!