Complex catalytic colloids on the basis of firefly luciferase as optical nanosensor platform. 2003

Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
Department of Biophysical M&O Science and Technologies, University of Genoa, Corso Europa 30, 16132 Genoa, Italy.

In the present work the layer-by-layer nano-assembly technique was used for the development of complex catalytic microparticles on the basis of firefly luciferase (FL). FL films containing 1, 2, or 3 monolayers were assembled on silver electrode QCM-resonators and on 520-nm diameter sulfonated polystyrene latex by alternate adsorption of FL and polycations using electrostatic interactions for the interlayer interaction. The assembly process was studied with quartz crystal microbalance, UV-vis spectroscopy, and microelectrophoresis (surface potential). Structural studies of the resulting multilayers confirmed stepwise deposition of FL and cationic poly(dimethyldiallyl ammonium chloride) with a bilayer thickness of 14 nm; a systematic shift of the surface potential from +28 mV for poly(dimethyldiallyl ammonium chloride) to -14 mV for luciferase outermost layer was established. The functionality and stability of the biocolloids were demonstrated by monitoring the intensity of the light emission. Factors influencing the light emitted upon catalytic activity of FL such as the number of luciferase layers in the film and polyion layer at the outermost layer were studied.

UI MeSH Term Description Entries
D008156 Luciferases Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates. Luciferase
D008863 Microspheres Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers. Latex Beads,Latex Particles,Latex Spheres,Microbeads,Bead, Latex,Beads, Latex,Latex Bead,Latex Particle,Latex Sphere,Microbead,Microsphere,Particle, Latex,Particles, Latex,Sphere, Latex,Spheres, Latex
D010783 Photometry Measurement of the various properties of light. Photometries
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D003102 Colloids Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other. Hydrocolloids,Colloid,Hydrocolloid
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001517 Coleoptera Order of winged insects also known as beetles comprising over 350,000 species in 150 families. They possess hard bodies with mouthparts adapted for chewing. Beetles,Beetle
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity

Related Publications

Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
November 1970, Biochemistry,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
September 1982, Archives of biochemistry and biophysics,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
December 2014, Journal of chemical theory and computation,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
November 1998, Biophysical journal,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
January 2004, Journal of biomedical optics,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
September 2011, Nature methods,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
July 2011, Chemistry, an Asian journal,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
July 1999, Nature structural biology,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
January 1991, Journal of bioluminescence and chemiluminescence,
Laura Pastorino, and Sandeep Disawal, and Claudio Nicolini, and Yuri M Lvov, and Victor V Erokhin
December 1970, Archives of biochemistry and biophysics,
Copied contents to your clipboard!