BIOMAT Database Logo BIOMAT Database Logo

Gaseous nitrogen oxides stimulate cell cycle progression by retinoblastoma phosphorylation via activation of cyclins/Cdks [correction]. 2003

Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Institute of Biochemistry, Chung Shan Medical University Hospital, Taichung, Taiwan.

Nitrogen oxides (NOx) are important indoor and outdoor air pollutants. Many studies have indicated that NOx gas causes lung tissue damage by its oxidation properties and its free radicals. In a previous study we demonstrated that NOx gas induced proliferation of human lung fibroblast MRC-5 cells. In this study we show that NOx gas stimulates MRC-5 cell proliferation by retinoblastoma (Rb) phosphorylation via activation of cyclin-cell division protein kinase (cdk) complexes [correction]. Western blot and immunoprecipitation data showed that NOx gas increased the expressions of cyclinA/cdk2, cyclinD1/cdk4, and cyclinE/cdk2 complexes in the cells at 9 h after treatment. The levels of phospho-Rb were also increased and cdk inhibitors (CKIs) p27 and p16 were apparently decreased. These data suggested that NOx gas stimulates cell-cycle progression by Rb phosphorylation via activation of cyclin-cdk complexes and inhibition of CKIs. In conclusion, the NOx-gas that induced lung fibroblast cell proliferation by stimulation of cell-cycle progression may contribute to lung fibrosis by NOx pollutants.

UI MeSH Term Description Entries
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D009589 Nitrogen Oxides Inorganic oxides that contain nitrogen. Nitrogen Oxide,Oxide, Nitrogen,Oxides, Nitrogen
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D005740 Gases The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Expression and activation of cdks (1 and 2) and cyclins in the cell cycle progression during liver regeneration.
January 1994, The Journal of biological chemistry,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Cdks, cyclins and CKIs: roles beyond cell cycle regulation.
August 2013, Development (Cambridge, England),
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
New insights into cyclins, CDKs, and cell cycle control.
June 2005, Seminars in cell & developmental biology,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Monoclonal antibodies specific for underphosphorylated retinoblastoma protein identify a cell cycle regulated phosphorylation site targeted by CDKs.
January 1997, Oncogene,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle.
February 2019, Cell reports,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Mammalian cell-cycle regulation: several Cdks, numerous cyclins and diverse compensatory mechanisms.
August 2009, Oncogene,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Cyclins regulating oocyte meiotic cell cycle progression†.
November 2019, Biology of reproduction,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Mammalian G1 cyclins and cell cycle progression.
July 1995, Proceedings of the Association of American Physicians,
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Inhibition of E2F1 activity and cell cycle progression by arsenic via retinoblastoma protein.
January 2017, Cell cycle (Georgetown, Tex.),
Jing-Hsien Chen, and Tsui-Hwa Tseng, and Yung-Chyan Ho, and Hui-Hsuan Lin, and Wea-Lung Lin, and Chau-Jong Wang
Analysis of site-specific phosphorylation of the retinoblastoma protein during cell cycle progression.
April 1999, Experimental cell research,
Copied contents to your clipboard!