Caenorhabditis elegans expressed sequence tags identify gene families and potential disease gene homologues. 1992

W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Maryland 20892.

A database containing mapped partial cDNA sequences from Caenorhabditis elegans will provide a ready starting point for identifying nematode homologues of important human genes and determining their functions in C. elegans. A total of 720 expressed sequence tags (ESTs) have been generated from 585 clones randomly selected from a mixed-stage C. elegans cDNA library. Comparison of these ESTs with sequence databases identified 422 new C. elegans genes, of which 317 are not similar to any sequences in the database. Twenty-six new genes have been mapped by YAC clone hybridization. Members of several gene families, including cuticle collagens, GTP-binding proteins, and RNA helicases were discovered. Many of the new genes are similar to known or potential human disease genes, including CFTR and the LDL receptor.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D015723 Gene Library A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences. DNA Library,cDNA Library,DNA Libraries,Gene Libraries,Libraries, DNA,Libraries, Gene,Libraries, cDNA,Library, DNA,Library, Gene,Library, cDNA,cDNA Libraries

Related Publications

W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
July 2008, BMC biology,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
January 2010, Advances in experimental medicine and biology,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
June 1994, Brain research. Molecular brain research,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
December 1994, Science (New York, N.Y.),
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
September 2001, Molecular and biochemical parasitology,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
January 1999, Annals of the New York Academy of Sciences,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
January 2001, Methods of biochemical analysis,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
March 1999, Human molecular genetics,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
July 1993, Nature genetics,
W R McCombie, and M D Adams, and J M Kelley, and M G FitzGerald, and T R Utterback, and M Khan, and M Dubnick, and A R Kerlavage, and J C Venter, and C Fields
July 1996, Biochemical and biophysical research communications,
Copied contents to your clipboard!