Biomaterial Database

Determination of hemoglobin Bart's in alpha thalassemia traits by two-site immunoradiometric assay. II. Detection of hemoglobin Bart's in alpha thalassemia traits. 1992

L Makonkawkeyoon, and S Ongchai, and T Sanguansermsri

Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand.

A sensitive method for the determination of minute amounts of Hb Bart's in hemolysate of alpha thalassemia-1 and alpha thalassemia-2 was carried out by two-site immunoradiometric assay. This technique is very sensitive and is able to detect minute amounts of substances. When this method is used with specific anti-Hb Bart's derived from affinity column chromatography, then labelled with radioactive 125I, the two-site immunoradiometric assay will contain both the sensitivity and specificity for the determination of Hb Bart's. In this study, parents of fetus with Hb Bart's hydrops fetalis contained high levels of Hb Bart's 0.73-1.11 per cent (mean +/- S.D. = 0.86 +/- 0.12%). Parents of Hb H diseases had Hb Bart's in two groups. The first group contained high levels of Hb Bart's of 0.73-1.20 per cent (mean +/- S.D. = 0.88 +/- 0.14%), while the second group had levels of 0.39-0.45 per cent (mean +/- S.D. = 0.42 +/- 0.02%). Mothers of patients with Hb H disease contained Hb Bart's of 0.42 +/- 0.48 per cent (mean +/- S.D. = 0.45 +/- 0.02%). Eight out of ten normal subjects contained low levels of Hb Bart's of 0.17-0.26 per cent (mean +/- S.D. = 0.22 +/- 0.04%), while two contained high levels of Hb Bart's of 0.46 and 0.43 per cent respectively. The two-site immunoradiometric assay was able to differentiate the level of Hb Bart's among alpha thalassemia-1, alpha thalassemia-2 and normal subjects.

UI	MeSH Term	Description	Entries
D006455	Hemoglobins, Abnormal	Hemoglobins characterized by structural alterations within the molecule. The alteration can be either absence, addition or substitution of one or more amino acids in the globin part of the molecule at selected positions in the polypeptide chains.	Abnormal Hemoglobins
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D015592	Immunoradiometric Assay	Form of radioimmunoassay in which excess specific labeled antibody is added directly to the test antigen being measured.	Assay, Immunoradiometric,Assays, Immunoradiometric,Immunoradiometric Assays
D017085	alpha-Thalassemia	A disorder characterized by reduced synthesis of the alpha chains of hemoglobin. The severity of this condition can vary from mild anemia to death, depending on the number of genes deleted.	Hemoglobin H Disease,Thalassemia-alpha,A-Thalassemia,Alpha Thalassemia,Disease, Hemoglobin H,Thalassemia alpha,Thalassemia, Alpha,alpha-Thalassemias