-transparent.png){kind=logo}
The complex formation between the ATP-analogous reactive dye Cibacron blue F3G-A and yeast phosphofructokinase is accompanied by a red shift of the visible absorption spectrum. From the position of lambdamax of the dye-phosphofructokinase complex in the polarity scales obtained from model solvents it may be concluded that the chromophoric system is evidently located in a highly apolaric range of the enzyme protein. The spectrophotometric titration of yeast phosphofructokinase with Cibacron blue F3G-A yielded a sigmoidal binding curve, which can be described by the MONOD-WYMAN-CHANGEUX model.
Related Publications
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
January 1984,
Biomedica biochimica acta,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
June 1972,
Journal of chromatography,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
December 1979,
Biochemical and biophysical research communications,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
April 1983,
European journal of biochemistry,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
July 1982,
Analytical biochemistry,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
January 1985,
Biomedica biochimica acta,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
August 1982,
The Biochemical journal,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
July 1980,
The Biochemical journal,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
May 1980,
Journal of chromatography,
H J Böhme, and
W Schellenberger, and
P Retterrath, and
E Hofmann, and
R Misselwitz
June 2010,
Colloids and surfaces. A, Physicochemical and engineering aspects,
