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We studied whether cultured rat pulmonary alveolar macrophages (PAM) could release glutathione (GSH) in response to latex beads, quartz, and crocidolite asbestos. PAM were exposed for 2 h to 0-100 micrograms of particles per 1 x 10(6) cells. Both quartz and asbestos produced concentration-dependent increases (up to 8-10-fold) in the amount of GSH recovered in the medium and decreases in the cellular GSH levels. In contrast, latex beads did not produce any changes in GSH levels. We also measured lactate dehydrogenase (LDH) levels as an index of toxicity. Only quartz and asbestos were able to produce increases in LDH levels in the medium. The release of GSH occurred at particle concentrations that did not cause release of LDH. Our results indicated that, in vitro, PAM release GSH in response to toxic particles.
D S Boehme, and
K R Maples, and
R F Henderson
September 1983,
Environmental health perspectives,
D S Boehme, and
K R Maples, and
R F Henderson
January 1988,
Respiration; international review of thoracic diseases,
D S Boehme, and
K R Maples, and
R F Henderson
September 1996,
American journal of respiratory and critical care medicine,
D S Boehme, and
K R Maples, and
R F Henderson
January 1986,
Annals of the New York Academy of Sciences,
D S Boehme, and
K R Maples, and
R F Henderson
September 2005,
American journal of respiratory cell and molecular biology,
D S Boehme, and
K R Maples, and
R F Henderson
August 1984,
Nihon Kyobu Shikkan Gakkai zasshi,
D S Boehme, and
K R Maples, and
R F Henderson
August 1979,
Chest,
D S Boehme, and
K R Maples, and
R F Henderson
September 1973,
Nature,
D S Boehme, and
K R Maples, and
R F Henderson
January 1985,
Journal of toxicology and environmental health,
D S Boehme, and
K R Maples, and
R F Henderson
July 2003,
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
