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To study the cellular mechanisms of interleukin-1 (IL-1) in the pituitary corticotroph, we studied the properties of IL-1 receptors on a mouse pituitary ACTH-producing cell line, AtT-20. Scatchard plot analysis revealed a single type of receptor with a Kd (dissociation constant) of 93 pM, and 482 binding sites/cell. [125I]IL-1 alpha binding competed with IL-1 alpha and IL-1 beta in an equimolar fashion. A 24 h pre-incubation with either CRH, epinephrine or nor-epinephrine increased the [125I]IL-1 alpha binding sites in the AtT-20 cells and conversely, a similar pre-incubation with either dexamethasone or tumour necrosis factor-alpha (TNF alpha) decreased them without affecting the affinity of the receptors in either case.
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
February 1995,
Neuroscience letters,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
November 1991,
Endocrinology,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
September 1994,
Brain research,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
January 2002,
Neuroimmunomodulation,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
November 1992,
Molecular and cellular endocrinology,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
May 2003,
British journal of pharmacology,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
September 1976,
Biochimica et biophysica acta,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
July 1989,
The Journal of endocrinology,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
October 1990,
Proceedings of the National Academy of Sciences of the United States of America,
H Kobayashi, and
J Fukata, and
T Tominaga, and
N Murakami, and
M Fukushima, and
O Ebisui, and
H Segawa, and
Y Nakai, and
H Imura
April 1984,
Molecular and cellular endocrinology,
