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Human umbilical vein endothelial cells were incubated with ATP, ADP, thrombin, or ionophore A23187 for as long as 600 seconds. A statistically significant rise in the prostaglandin I2 (prostacyclin; PGI2) and thromboxane A2 (TxA2) release was observed after 45 seconds. The maximum amount of cytosolic free Ca2+ was reached between 20 and 30 seconds. A statistically significant elevation of intracellular adenosine 3',5'-cyclic monophosphate (cAMP) levels was observed only in response to ATP and ADP after 120 seconds. Furthermore, adenosine, caffeine, phorbol 12-myristate 13-acetate (PMA), and adenosine 5'-(alpha, beta-methylene)triphosphate were tested alone or in combination with ATP or thrombin. PMA inhibited ATP-stimulated eicosanoid biosynthesis but had no effect on thrombin. Of the agents used to increase cytosolic free Ca2+ concentrations, only PMA failed to provoke eicosanoid release. Similarly, only PMA failed to induce a Ca2+ flux in the absence of extracellular Ca2+. The data presented show that PGI2 and TxA2 release from human umbilical cord endothelial cells is closely associated with Ca2+ mobilized from intracellular sources. Extracellular Ca2+, as well as changes in intracellular cAMP levels, has no influence on endothelial eicosanoid synthesis, at least for short-term regulation (less than or equal to 600 seconds).
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
September 1977,
Proceedings of the National Academy of Sciences of the United States of America,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
January 1996,
Life sciences,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
September 1989,
The Journal of pharmacology and experimental therapeutics,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
January 1987,
Advances in prostaglandin, thromboxane, and leukotriene research,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
April 1987,
Japanese circulation journal,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
October 2002,
The Journal of rheumatology,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
October 1983,
Biochemical pharmacology,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
December 1978,
British journal of pharmacology,
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
November 1993,
Arerugi = [Allergy],
A Griesmacher, and
G Weigel, and
M David, and
G Horvath, and
M M Mueller
June 1986,
Changgeng yi xue za zhi,
