Biomaterial Database

Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey. 1992

K Eimert, and F Siegemund

Dept. of Genetics, Martin Luther University, Halle/S., Germany.

The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.

UI	MeSH Term	Description	Entries
D010770	Phosphotransferases	A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.	Kinases,Phosphotransferase,Phosphotransferases, ATP,Transphosphorylase,Transphosphorylases,Kinase,ATP Phosphotransferases
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D001937	Brassica	A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).	Broccoli,Brussel Sprout,Cabbage,Cauliflower,Collard Green,Kale,Cabbages,Collard Greens
D012231	Rhizobium	A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
D014162	Transfection	The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.	Transfections
D014170	Transformation, Genetic	Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.	Genetic Transformation,Genetic Transformations,Transformations, Genetic
D019868	Kanamycin Kinase	A class of enzymes that inactivate aminocyclitol-aminoglycoside antibiotics (AMINOGLYCOSIDES) by regiospecific PHOSPHORYLATION of the 3' and/or 5' hydroxyl.	Aminoglycoside Phosphotransferase,Neomycin Phosphotransferase,APH(3')-IIIa,APH(3')-IIb,APHVII,APT-3'-I and II,Amikacin 3'-Phosphotransferase,Aminocyclitol Phosphotransferase,Aminoglycoside 3'-Phosphotransferase Type VIII,Aminoglycoside 3'-Phosphotransferases (I and II),Aminoglycoside Phosphotransferase Type III,Kanamycin-Neomycin Phosphate Transferase,Neomycin Phosphotransferase II,aphVII Gene Product,3'-Phosphotransferase, Amikacin,APT 3' I and II,Amikacin 3' Phosphotransferase,Aminoglycoside 3' Phosphotransferase Type VIII,Kanamycin Neomycin Phosphate Transferase,Kinase, Kanamycin,Phosphate Transferase, Kanamycin-Neomycin,Phosphotransferase II, Neomycin,Phosphotransferase, Aminocyclitol,Phosphotransferase, Aminoglycoside,Phosphotransferase, Neomycin,Transferase, Kanamycin-Neomycin Phosphate