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Bovine rotavirus T449 was isolated from feces of a calf with diarrhea. Serological characterization by serotype-specific monoclonal antibodies showed that the T449 virus belonged to serotype 1. This is the first report of a bovine rotavirus that does not belong to serotype 6, 8, or 10. The serotype 1 designation was confirmed by using an immunoperoxidase focus neutralization assay. The gene encoding the major neutralization antigen (VP7) was cloned and its nucleotide sequence was determined. The sequence obtained was 1062 bp in length and contained an open reading frame corresponding to 326 amino acid residues. Comparative analysis of the deduced amino acid sequence with the corresponding sequence of the human serotype 1 rotavirus strain, Wa, revealed a 90% identity. When compared to the predicted amino acid sequence of VP7 protein of the other serotypes an overall divergence of 20 to 25% was detected. These data show that the serological typing agrees with the result of the genetic analysis.
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
August 1990,
Nucleic acids research,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
January 1988,
Nucleic acids research,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
July 1983,
Nucleic acids research,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
November 1986,
The Journal of general virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
May 1988,
Journal of virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
April 1987,
Journal of virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
February 1989,
Virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
December 1991,
Virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
March 1985,
Virology,
J Blackhall, and
R Bellinzoni, and
N Mattion, and
M K Estes, and
J L La Torre, and
G Magnusson
January 1997,
Advances in experimental medicine and biology,
