BIOMAT Database Logo BIOMAT Database Logo

Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis. 1992

A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Department of Biology, Vrije Universiteit, Amsterdam, The Netherlands.

A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chain, however, in this case connected by two distinct C peptide, C alpha and C beta, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the C alpha peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron: the growth controlling neuroendocrine light green cells of the Lymnaea CNS.

UI MeSH Term Description Entries
D007448 Invertebrates Animals that have no spinal column. Brachiopoda,Mesozoa,Brachiopodas,Invertebrate,Mesozoas
D008195 Lymnaea A genus of dextrally coiled freshwater snails that includes some species of importance as intermediate hosts of parasitic flukes. Lymnea,Lymnaeas,Lymneas
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009479 Neuropeptides Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells. Neuropeptide
D011498 Protein Precursors Precursors, Protein
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Characterization of a cDNA clone encoding molluscan insulin-related peptide II of Lymnaea stagnalis.
August 1991, European journal of biochemistry,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Expression and characterization of molluscan insulin-related peptide VII from the mollusc Lymnaea stagnalis.
January 1996, Neuroscience,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Characterization of a cDNA clone encoding multiple copies of the neuropeptide APGWamide in the mollusk Lymnaea stagnalis.
May 1992, The Journal of neuroscience : the official journal of the Society for Neuroscience,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Evolutionary conservation of the insulin gene structure in invertebrates: cloning of the gene encoding molluscan insulin-related peptide III from Lymnaea stagnalis.
August 1993, Journal of molecular endocrinology,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Purification and sequencing of molluscan insulin-related peptide II from the neuroendocrine light green cells in Lymnaea stagnalis.
June 1992, Endocrinology,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Differential expression of four genes encoding molluscan insulin-related peptides in the central nervous system of the pond snail Lymnaea stagnalis.
July 1992, Cell and tissue research,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Purification and sequencing of molluscan insulin-related peptide I (MIP I) from the neuroendocrine light green cells of Lymnaea stagnalis.
May 1992, Molecular and cellular endocrinology,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Evolution of the vasopressin/oxytocin superfamily: characterization of a cDNA encoding a vasopressin-related precursor, preproconopressin, from the mollusc Lymnaea stagnalis.
May 1992, Proceedings of the National Academy of Sciences of the United States of America,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Characterization of a putative molluscan insulin-related peptide receptor.
September 1995, Gene,
A B Smit, and S F Thijsen, and W P Geraerts, and I Meester, and H van Heerikhuizen, and J Joosse
Isolation, characterization, and evolutionary aspects of a cDNA clone encoding multiple neuropeptides involved in the stereotyped egg-laying behavior of the freshwater snail Lymnaea stagnalis.
November 1988, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Copied contents to your clipboard!