Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. 1992

A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
Department of Medicine, Harvard Medical School, Massachusetts General Hospital, Boston 02114.

A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. The dissociation constants (Kd) for the CTRs on these BIN-67 cells is approximately 0.42 nM for salmon CT and approximately 4.6 nM for human CT. To clone a human CTR (hCTR), a BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently cloned. One positive clone of 3,588 bp was identified. Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). The expressed hCTR was coupled to adenylate cyclase. Northern analysis with the hCTR cDNA probe indicated a single transcript of approximately 4.2 kb. The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. The amino acid sequence of the hCTR is 73% identical to the pCTR, although the hCTR contains an insert of 16 amino acids between transmembrane domain I and II. The structural differences may account for observed differences in binding affinity between the porcine renal and human ovarian CTRs. The CTRs are closely related to the receptors for parathyroid hormone-parathyroid hormone-related peptide and secretin; these receptors comprise a distinct family of G protein-coupled seven transmembrane domain receptors. Interestingly, the hCTR sequence is remotely related to the cAMP receptor of Dictyostelium discoideum (21% identical), but is not significantly related to other G protein-coupled receptor sequences now in the data bases.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D011953 Receptors, Cyclic AMP Cell surface proteins that bind cyclic AMP with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized cyclic AMP receptors are those of the slime mold Dictyostelium discoideum. The transcription regulator CYCLIC AMP RECEPTOR PROTEIN of prokaryotes is not included nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES. Cyclic AMP Receptors,cAMP Receptors,Cyclic AMP Receptor,Receptors, cAMP,cAMP Receptor,Receptor, Cyclic AMP,Receptor, cAMP
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
August 1997, Journal of neurochemistry,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
November 1978, Cancer,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
August 1994, Molecular pharmacology,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
July 1990, Gynecologic oncology,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
February 1993, Gynecologic oncology,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
May 1988, Gynecologic oncology,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
September 1991, Acta endocrinologica,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
January 1983, Nihon Sanka Fujinka Gakkai zasshi,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
February 1989, Nihon Sanka Fujinka Gakkai zasshi,
A H Gorn, and H Y Lin, and M Yamin, and P E Auron, and M R Flannery, and D R Tapp, and C A Manning, and H F Lodish, and S M Krane, and S R Goldring
August 1995, Gynecologic oncology,
Copied contents to your clipboard!