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We have synthesized a series of peptides, which cover almost the whole range of the N-terminal extracellular domain of human 55kDa TNF receptor (55kDa TNF-R). The peptides were examined for the binding activity to TNF by solid phase binding assay and for the inhibition of TNF cytotoxicity to mouse L-M cells. The peptide 159-178 exhibited remarkably higher binding activity to TNF than other peptides did. The specificity of the TNF binding to the peptides was confirmed by their inability to bind other cytokines. The peptide 159-178 also inhibited TNF cytotoxicity. These results indicate that the specific binding site of 55kDa TNF-R to TNF might reside within the peptide segment of amino acid numbers 159 to 178 in the N-terminal extracellular domain.
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
April 2004,
Current opinion in structural biology,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
January 2024,
Veterinary sciences,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
December 1994,
The Journal of infectious diseases,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
July 2002,
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
November 1992,
The Journal of biological chemistry,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
November 2003,
The Journal of biological chemistry,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
July 1992,
Archives of biochemistry and biophysics,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
May 2007,
The journal of investigative dermatology. Symposium proceedings,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
March 1987,
Journal of leukocyte biology,
B L Lie, and
D Tunemoto, and
H Hemmi, and
Y Mizukami, and
H Fukuda, and
H Kikuchi, and
S Kato, and
N Numao
June 1999,
The Journal of experimental medicine,
