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Specific inhibitors of the endoplasmic-reticulum Ca2+ pump will deplete intracellular stores and are therefore useful to study the role of store depletion on plasma-membrane Ca2+ permeability. We now report that the Ca(2+)-pump inhibitor 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tBuBHQ) reduces the passive Ca2+ leak from the internal stores in permeabilized A7r5 vascular smooth-muscle cells. This aspecific effect occurred at concentrations that are normally used to empty the stores in intact cells. Cyclopiazonic acid exerted a similar, although less pronounced effect, while thapsigargin did not affect the passive Ca2+ leak. The inositol 1,4,5-trisphosphate-mediated Ca2+ release was not affected. tBuBHQ and cyclopiazonic acid cannot therefore be used as specific tools to probe the mechanism of receptor-mediated Ca2+ entry.
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
November 1997,
Japanese journal of pharmacology,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
December 1992,
Journal of biochemistry,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
November 1987,
FEBS letters,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
November 1990,
FEBS letters,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
January 1990,
Free radical research communications,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
January 1994,
The Journal of physiology,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
August 2015,
Cell calcium,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
September 1989,
The Journal of biological chemistry,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
May 1992,
Agents and actions,
L Missiaen, and
H De Smedt, and
G Droogmans, and
R Casteels
June 1992,
FEBS letters,
