Biomaterial Database

[Use of culture media with sheep serum for maintenance and isolation of Leptospira strains]. 1976

M Nicolescu, and G Moldoveanu

A study was carried out on the effects of a culture medium prepared with sheep serum inactivated at 68 degrees C on Leptospira cultures. Good results were obtained showing that the medium with sheep serum can be used for the cultivation of Leptospira in view of the preparation of antigens for microscopic agglutination and complement fixation. In experiments on the isolation of germs from organocultures and haemocultures the proportion of positive results obtained with sheep serum was smaller than with the media containing rabbit serum.

UI	MeSH Term	Description	Entries
D007919	Leptospira	A genus of aerobic, helical spirochetes, some species of which are pathogenic, others free-living or saprophytic.
D001769	Blood	The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli