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With regard to the legal regulations for the diagnosis of Aujeszky's disease in pigs, a retrospective immunoenzymatic study was performed on brains of 20 pigs, in which Aujeszky's disease had been diagnosed based only on clinical, macroscopical and histological findings, although the etiological agent could not be demonstrated neither by immunofluorescent technique nor by virological cultivation. Applying the peroxidase-antiperoxidase-(PAP) method, in 6 of 20 animals (30%) viral antigen of porcine herpesvirus type 1 was demonstrated in situ in at least two of five different locations of the brain. Reasons for negative results in immunohistological and virological investigations are discussed and the significance of the performed immunoenzymatic technique is pointed out.
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G Matschullat, and
B Röder, and
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J Pohlenz
January 1994,
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P Wohlsein, and
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G Matschullat, and
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J Pohlenz
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P Wohlsein, and
A Klimke, and
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P Wohlsein, and
A Klimke, and
G Matschullat, and
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J Pohlenz
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P Wohlsein, and
A Klimke, and
G Matschullat, and
B Röder, and
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J Pohlenz
November 1989,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
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G Matschullat, and
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J Pohlenz
September 1981,
DTW. Deutsche tierarztliche Wochenschrift,
P Wohlsein, and
A Klimke, and
G Matschullat, and
B Röder, and
B M Rim, and
J Pohlenz
September 1978,
The Veterinary record,
P Wohlsein, and
A Klimke, and
G Matschullat, and
B Röder, and
B M Rim, and
J Pohlenz
January 1988,
DTW. Deutsche tierarztliche Wochenschrift,
P Wohlsein, and
A Klimke, and
G Matschullat, and
B Röder, and
B M Rim, and
J Pohlenz
January 1983,
Tierarztliche Praxis,
P Wohlsein, and
A Klimke, and
G Matschullat, and
B Röder, and
B M Rim, and
J Pohlenz
January 1994,
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993),
