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The stable transfection of immortalized Rat-1 and rat skin primary fibroblast cell lines by calcium phosphate precipitation, lipofection and electroporation methods have been examined. The lipofection method was found to be better than the other methods in terms of higher transfection efficiency and convenient use. Expression of beta-galactosidase from two different viral promoters showed that the level of transgene expression depends on the promoter strength in a particular cell type. The results presented here show that the transgene expression is extremely variable among different colonies generated from individually transfected cells. Therefore, it is necessary to examine individual colonies of cells for the production of reporter gene to obtain cell lines expressing high amounts of gene products.
J Ray, and
F H Gage
January 1993,
Methods in enzymology,
J Ray, and
F H Gage
January 1991,
Methods in molecular biology (Clifton, N.J.),
J Ray, and
F H Gage
January 1998,
Cell transplantation,
J Ray, and
F H Gage
January 1990,
Biology of the cell,
J Ray, and
F H Gage
July 1990,
Journal of neuroscience research,
J Ray, and
F H Gage
October 1996,
Journal of medical virology,
J Ray, and
F H Gage
January 2003,
Methods in molecular medicine,
J Ray, and
F H Gage
August 1989,
Blood,
J Ray, and
F H Gage
February 2019,
In vitro cellular & developmental biology. Animal,
J Ray, and
F H Gage
January 1994,
Gene therapy,
