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By using the polymerase chain reaction (PCR) we detected latent herpes simplex virus type 1 (HSV-1) in human geniculate and trigeminal ganglia obtained from autopsy cases. A pair of primers which were specific for a part of the HSV-1 thymidine kinase domain were used for detection of HSV DNA. We also examined the latency-associated transcript (LAT), known as latency-specific RNA, by means of reverse transcription-PCR with a pair of LAT-specific primers. HSV-1 DNA was detected in 16 of 17 (94%) trigeminal ganglia and in 15 to 17 (88%) geniculate ganglia of adults. We also demonstrated HSV-1 RNA derived from the LAT in both types of ganglia. These findings suggest that HSV-1 latently infects the majority of geniculate and trigeminal ganglia of adults, and that PCR and reverse transcription-PCR are useful tools for analysis of HSV latency.
T Takasu, and
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K C Sato, and
S Fukuda, and
Y Inuyama, and
K Nagashima
February 1994,
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T Takasu, and
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S Fukuda, and
Y Inuyama, and
K Nagashima
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T Takasu, and
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S Fukuda, and
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K Nagashima
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T Takasu, and
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S Fukuda, and
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K Nagashima
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T Takasu, and
Y Furuta, and
K C Sato, and
S Fukuda, and
Y Inuyama, and
K Nagashima
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T Takasu, and
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S Fukuda, and
Y Inuyama, and
K Nagashima
September 1996,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
T Takasu, and
Y Furuta, and
K C Sato, and
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Y Inuyama, and
K Nagashima
January 1997,
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T Takasu, and
Y Furuta, and
K C Sato, and
S Fukuda, and
Y Inuyama, and
K Nagashima
July 1996,
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T Takasu, and
Y Furuta, and
K C Sato, and
S Fukuda, and
Y Inuyama, and
K Nagashima
April 1993,
Journal of virology,
T Takasu, and
Y Furuta, and
K C Sato, and
S Fukuda, and
Y Inuyama, and
K Nagashima
January 1990,
Medical microbiology and immunology,
