BIOMAT Database Logo BIOMAT Database Logo

Enteric adenovirus type 41 isolates: cloning, physical maps and diversity in restriction enzyme cleavage pattern. 1992

Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Department of Molecular Biology, Cancer Research Institute, Hokkaido, Japan.

Adenovirus (Ad) type 40 and 41 DNAs were directly extracted from stool specimens of children with gastroenteritis. Two new strains of Ad41, Sanekata and Ehime strain, were cloned and their restriction maps were constructed. The left terminal end of the cloned Ad41 genome, EcoRI-E fragment of the Sanekata strain and EcoRI-F fragment of the Ehime strain, had transforming ability in rat 3Y1 cells. Only one of the 35 isolates of Ad40 tested showed a different restriction profile, while three different restriction profiles were found in DNAs from Ad41 isolates.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002472 Cell Transformation, Viral An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus. Transformation, Viral Cell,Viral Cell Transformation,Cell Transformations, Viral,Transformations, Viral Cell,Viral Cell Transformations
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003968 Diarrhea, Infantile DIARRHEA occurring in infants from newborn to 24-months old. Infantile Diarrhea,Diarrheas, Infantile,Infantile Diarrheas
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000258 Adenovirus Infections, Human Respiratory and conjunctival infections caused by 33 identified serotypes of human adenoviruses. Pharyngo-Conjunctival Fever,Human Adenovirus Infections,Infections, Human Adenovirus,Adenovirus Infection, Human,Fever, Pharyngo-Conjunctival,Human Adenovirus Infection,Infection, Human Adenovirus,Pharyngo Conjunctival Fever
D000260 Adenoviruses, Human Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G. APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses

Related Publications

Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Physical organization of the enteric adenovirus type 41 early region 1A.
May 1988, Virology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Restriction enzyme cleavage maps of the DNA of two cauliflower mosaic virus isolates.
May 1980, Virology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Restriction analysis of the prototype strain of enteric adenovirus type 41 using exonuclease III.
July 1992, Journal of virological methods,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Molecular cloning and restriction enzyme analysis of bovine adenovirus type 3.
January 1992, Intervirology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Structural studies of human enteric adenovirus type 41.
February 2002, Virology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Cleavage maps of human adenovirus type 7 DNA by restriction endonuclease HindIII and EcoRI.
October 1977, Virology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Restriction enzyme maps for equine adenovirus 1 genome.
June 1992, Veterinary microbiology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Molecular cloning and restriction enzyme mapping of avian adenovirus type 8 DNA.
December 1996, Virus research,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Enzyme-linked immunosorbent assay for detection of enteric adenovirus 41.
September 1985, Journal of medical virology,
Y Yamashita, and T Hotsubo, and S Nakata, and T Yamaguchi, and T Sanekata, and S Chiba, and M Homma, and K Fujinaga
Block in entry of enteric adenovirus type 41 in HEK293 cells.
March 2011, Virus research,
Copied contents to your clipboard!