-transparent.png){kind=logo}
1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] inhibits lymphocyte proliferation and production of antibodies and lymphokines such as interleukin (IL)-2 and interferon gamma. These lymphocyte functions are dependent upon cytokines, including IL-1 alpha, IL-1 beta, IL-6 and tumour necrosis factor alpha (TNF-alpha), produced by the antigen presenting cells. In the present study we examined the effect of 1,25-(OH)2D3 on the production of these cytokines, as well as superoxide generation by freshly isolated mononuclear cells and partially purified monocytes. The immediate precursor of 1,25(OH)2D3, 25-OH D3, and the synthetic analogue MC 903 ('Calcipotriol') were examined in parallel. 1,25-(OH)2D3 dose-dependently inhibited the production of IL-alpha, IL-6 and TNF-alpha by Escherichia coli lipopolysaccharide (LPS)-stimulated monocytes, without affecting superoxide production. MC 903 had comparable effects while 25-OH D3 was ineffective. The inhibition caused by 1,25-(OH)2D3 was not abolished by supraoptimal concentrations of LPS or indomethacin. 1,25-(OH)2D3 had similar effects on secreted and cell-associated IL-alpha. Nuclear run-off analysis indicated that inhibition of these cytokines was not caused by impaired production of mRNA. Taken together, the study demonstrates a vitamin D-induced inhibitory effect of LPS-driven monokine production, which is most likely a vitamin D-receptor mediated phenomenon exerted at a post-transcriptional, presecretory level. Impaired monokine production may be of importance in 1,25-(OH)2D3-mediated inhibition of lymphocyte functions in vitro.
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
March 2004,
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
September 1983,
The Biochemical journal,
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
March 1974,
Science (New York, N.Y.),
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
December 2006,
Biology of reproduction,
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
February 1986,
Journal of immunology (Baltimore, Md. : 1950),
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
February 1992,
Immunology,
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
May 2016,
G3 (Bethesda, Md.),
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
August 1986,
The Journal of clinical investigation,
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
September 2020,
Medicina (Kaunas, Lithuania),
K Müller, and
P M Haahr, and
M Diamant, and
K Rieneck, and
A Kharazmi, and
K Bendtzen
March 1985,
Endocrinology,
