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Methods are described by which cryopreserved cells can be utilized in a number of in vitro assays. On a per cell basis, nearly total recovery of function can be demonstrated for lymphocyte transformation (mitogens, antigens and MLC) and rosette-forming cells. Excellent recovery of mononuclear cell production of leukocyte migration inhibitory factor was also observed. Highly reproducible activity was recovered on a per lymphocyte basis in lymphocyte cytotoxicity but with a definite decrement in the percentage recovery. Both for longitudinal studies of immune function and for standardization of these assays in one or more laboratories such cryopreserved cells are ofimmense value and should be widely utilized.
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 1992,
Immunopharmacology and immunotoxicology,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
June 2003,
Current protocols in immunology,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 1979,
Biomaterials, medical devices, and artificial organs,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
December 1978,
National Cancer Institute monograph,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 1978,
Annals of surgery,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
November 2003,
Current protocols in immunology,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 2014,
Frontiers in oncology,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 1977,
Chemico-biological interactions,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
June 1977,
Transplantation,
R K Oldham, and
J H Dean, and
G B Cannon, and
J R Ortaldo, and
G Dunston, and
F Applebaum, and
J L McCoy, and
J Djeu, and
R B Herberman
January 1970,
International archives of allergy and applied immunology,
