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Active immunization in hamsters with live Entamoeba histolytica trophozoites of low virulence. 1992
V Tsutsumi, and
M Shibayama-Salas, and
R Campos-Rodríguez, and
J Pacheco-Yépez, and
A Martínez-Palomo
Departamento de Patología Experimental, Centro de Investigación de Estudios Avanzados del IPN, México, DF, México.
Live trophozoites of Entamoeba histolytica of low virulence (LV) were inoculated intraportally in hamsters. One week later they were challenged intrahepatically with highly virulent (HV) trophozoites of the same strain and sacrificed 1 week later. Significant protection manifested by the production of smaller lesions was observed in immunized animals. Antiamebic IgG levels were significantly higher in the immunized group. Histologically, livers of immunized animals presented small lesions containing foamy macrophages, epithelioid cells, fibroblasts and devoid of amebas. Lymphocyte and plasma cell infiltrates were seen periportally.
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