Periodontal bone loss in mice orally inoculated with Peptostreptococcus anaerobius, Pept. magnus and Actinobacillus actinomycetemcomitans was compared to that in sham-inoculated mice. Six-to-8-week-old BALB/c mice were inoculated with 1 x 10(5), 1 x 10(7) or 1 x 10(9) colony-forming units (c.f.u.) of bacteria in 50 microliters of medium. Ten mice received each concentration of bacteria and 10 sham-inoculated mice acted as controls. Five mice from each of the groups were killed 6 weeks after inoculation and the remaining five mice at 12 weeks. Right hemimandibles were defleshed, stained and bone loss was measured using an image analyser. All the organisms tested were associated with bone loss. Animals that had received Pept. anaerobius and Pept. magnus had up to 18% more bone loss than those sham inoculated. In contrast, mice inoculated with A. actinomycetemcomitans had up to 38% more bone loss than the sham-inoculated animals, this amount of loss occurring at the lowest inoculation of 1 x 10(5) c.f.u. These data demonstrate a differential ability of micro-organisms to cause periodontal bone loss in mice.