-transparent.png){kind=logo}
The region between tumor necrosis factor (TNF) and HLA-B in the central major histocompatibility complex (MHC) is polymorphic and associated with several autoimmune diseases. The polymorphisms are haplospecific or haplotypic and retained within the same MHC ancestral haplotype (AH). We have cloned this region from four AHs into lambda bacteriophage and found that a highly polymorphic region in the TNF-HLA-B interval is duplicated. Clones from this region isolated from three MHC AHs show two populations. The regions, designated CL1 and CL2, have different sizes of Bam HI fragments carrying the duplicated sequences. These fragments correspond to those seen after Bam HI restriction fragment length polymorphism (RFLP) analysis of genomic DNA from the same cell lines. Pulsed field gel electrophoresis analysis shows that both CL1 and CL2 are in the central MHC and are about 16 kilobases apart. DNA cloning and RFLP analysis demonstrate that the CL region is highly polymorphic but retained within an MHC AH. Polymorphism and duplication are common characteristics of the genes found in the MHC and therefore the CL sequences have the potential to be interesting in this respect.
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
March 1999,
Journal of molecular evolution,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
January 2009,
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
February 2005,
Molecular genetics and genomics : MGG,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
February 1993,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
January 1989,
Proceedings of the National Academy of Sciences of the United States of America,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
April 1994,
Yeast (Chichester, England),
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
February 2001,
Genome research,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
June 2018,
International journal of immunogenetics,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
August 1998,
Proceedings of the National Academy of Sciences of the United States of America,
C Leelayuwat, and
L J Abraham, and
H Tabarias, and
F T Christiansen, and
R L Dawkins
January 1999,
The Plant cell,
