Biomaterial Database

Restriction fragment length polymorphism analysis of Listeria monocytogenes and its application to epidemiological investigations. 1992

A E Lew, and P M Desmarchelier

Tropical Health Program, University of Queensland, Brisbane, Australia.

The restriction fragment length polymorphisms (RFLPs) of 64 random and potentially related strains of Listeria monocytogenes were analysed and compared using a probe comprised of two L. monocytogenes chromosome fragments cloned into a lambda vector. Twelve RFLP types were defined using 14 isolates of clinical origin, 42 food isolates and eight food associated environmental strains. Of the RFLP types, some were common to a particular serovar and source, whereas others were widespread amongst all serovars and sources. One of the two most common RFLP patterns was associated with serovar 1/2 isolates from food or the environment, whereas another dominant pattern was associated most commonly with serovar four isolates from all sources. The potential relationships between epidemiologically related strains were examined, with the analysis of types from a suspected listeriosis outbreak, from clinical maternal-foetal cases, and from an ice-cream factory environmental study. Serotyping alone was not a sufficient marker for the comparison of these strains whereas further discrimination of strains was possible with RFLP analysis.

UI	MeSH Term	Description	Entries
D008088	Listeriosis	Infections with bacteria of the genus LISTERIA.	Listeria Infections,Infections, Listeria,Infection, Listeria,Listeria Infection,Listerioses
D008089	Listeria monocytogenes	A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D005516	Food Microbiology	The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.	Microbiology, Food
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species
D015373	Bacterial Typing Techniques	Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.	Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin