T cell maturation stage-linked heterogeneity of the glycosylphosphatidylinositol membrane anchor of Thy-1. 1992

I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
Department of Immunology, Nagoya University School of Medicine, Aichi, Japan.

We showed that some of Thy-1 molecules on murine thymocytes are resistant to phosphatidylinositol-specific phospholipase C (PI-PLC) derived from Bacillus thuringiensis. Both immature thymocytes with low CD3 expression and mature thymic T lymphocytes with high CD3 expression carried the PI-PLC-resistant Thy-1, and the PI-PLC-sensitivity of Thy-1 extensively varied among thymocyte subpopulations. In contrast, the same PI-PLC fully hydrolysed the anchor of Thy-1 on peripheral T lymphocytes. When the latter cells were activated with mitogen in vitro, however, some Thy-1 on them became resistant to PI-PLC. We then found that virtually all Thy-1 molecules on thymocytes became sensitive to PI-PLC when they were treated with hydroxylamine that should cleave ester-linked lipids. The result ruled out the possibility that the PI-PLC-resistant Thy-1 had a transmembranous peptide sequence, and suggested the presence of an additional fatty acyl group on the inositol ring of the Thy-1 anchor. In addition, the molecular size of the PI-PLC-resistant membrane-bound Thy-1 was only marginally larger than that of the PI-PLC-sensitive solubilized Thy-1 in detergent-partitioning SDS-PAGE analysis.

UI MeSH Term Description Entries
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008809 Mice, Inbred C3H An inbred strain of mouse that is used as a general purpose strain in a wide variety of RESEARCH areas including CANCER; INFECTIOUS DISEASES; sensorineural, and cardiovascular biology research. Mice, C3H,Mouse, C3H,Mouse, Inbred C3H,C3H Mice,C3H Mice, Inbred,C3H Mouse,C3H Mouse, Inbred,Inbred C3H Mice,Inbred C3H Mouse
D010727 Phosphoric Diester Hydrolases A class of enzymes that catalyze the hydrolysis of one of the two ester bonds in a phosphodiester compound. EC 3.1.4. Phosphodiesterase,Phosphodiesterases,Hydrolases, Phosphoric Diester
D006898 Hydroxylamines Organic compounds that contain the (-NH2OH) radical.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000954 Antigens, Surface Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated. Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D017261 Glycosylphosphatidylinositols Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes. GPI Membrane Anchor,GPI Membrane Anchors,Glycosyl-Phosphatidylinositol Membrane Protein Anchor,Glycosylated Phosphatidylinositol,Glycosylphosphatidylinositol Anchor,Glycosylphosphatidylinositol Anchors,Phosphatidylinositol Glycan,Gly-PtdIns,Glycoinositol Phospholipid Membrane Anchor,Glycosyl-Phosphatidylinositol,Glycosyl-Phosphatidylinositol Membrane Protein Anchors,Glycosylated Phosphatidylinositols,Glycosylphosphatidylinositol,Glycosylphosphatidylinositol Linkage,PI-Glycan,Anchor, GPI Membrane,Anchor, Glycosylphosphatidylinositol,Anchors, GPI Membrane,Anchors, Glycosylphosphatidylinositol,Glycan, Phosphatidylinositol,Glycosyl Phosphatidylinositol,Glycosyl Phosphatidylinositol Membrane Protein Anchor,Glycosyl Phosphatidylinositol Membrane Protein Anchors,Linkage, Glycosylphosphatidylinositol,Membrane Anchor, GPI,Membrane Anchors, GPI,PI Glycan,Phosphatidylinositol, Glycosylated,Phosphatidylinositols, Glycosylated
D043265 Phosphatidylinositol Diacylglycerol-Lyase A phosphorus-oxygen lyase found primarily in BACTERIA. The enzyme catalyzes the cleavage of a phosphoester linkage in 1-phosphatidyl-1D-myo-inositol to form 1D-myo-inositol 1,2-cyclic phosphate and diacylglycerol. The enzyme was formerly classified as a phosphoric diester hydrolase (EC 3.1.4.10) and is often referred to as a TYPE C PHOSPHOLIPASES. However it is now known that a cyclic phosphate is the final product of this enzyme and that water does not enter into the reaction. 1-Phosphatidylinositol Phosphodiesterase,Monophosphatidylinositol Phosphodiesterase,1 Phosphatidylinositol Phosphodiesterase,Diacylglycerol-Lyase, Phosphatidylinositol,Phosphatidylinositol Diacylglycerol Lyase,Phosphodiesterase, 1-Phosphatidylinositol,Phosphodiesterase, Monophosphatidylinositol

Related Publications

I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
December 1991, The Journal of biological chemistry,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
May 1999, Journal of immunology (Baltimore, Md. : 1950),
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
July 1991, The Journal of biological chemistry,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
January 1995, Acta haematologica Polonica,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
April 1994, Seminars in immunology,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
August 1991, European journal of immunology,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
June 1988, Biochemical Society transactions,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
January 1999, Biochemistry and cell biology = Biochimie et biologie cellulaire,
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
September 2004, Journal of immunology (Baltimore, Md. : 1950),
I Nakashima, and T Yoshida, and Y H Zhang, and M Y Pu, and R Taguchi, and H Ikezawa, and K Isobe, and T Iwamoto, and M Kato, and T Lwin
November 1994, Bioorganic & medicinal chemistry,
Copied contents to your clipboard!