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Yoshida ascites tumor cells were synchronized in suspension cultures with thymidine in excess or with fluordeoxyuridine. Cells were washed after incubation with thymidine or treated with thymidine after incubation with fluordeoxyuridine. 2 h after terminating the S phase blockade 74--80% of the cells are S phase cells, 6 h later 70--80% of the cells had accumulated in the G 2 phase. Cultures enriched with cells of the S phase or the G 2 phase were treated with Trenimon. Increase of cell numbers within the following 48 h was inhibited in both cases in the same order of magnitude. In addition cells in various phases of growth were separated by density gradient zentrifugation and treated with Trenimon. Again cell numbers after 48 h were identical.
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