BIOMAT Database Logo BIOMAT Database Logo

The effect of interferon gamma on epidermal growth factor receptor expression in normal and malignant ovarian epithelial cells. 1992

M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC 27710.

OBJECTIVE We examined the effect of interferon gamma on proliferation and epidermal growth factor receptor expression in ovarian cancer cell lines and normal ovarian epithelial cells. METHODS The tritiated thymidine incorporation assay was used to assess the effect of interferon gamma on proliferation. Scatchard analysis of anti-epidermal growth factor receptor antibody binding, and Western blotting of immunoprecipitates was used to assess the effect of interferon gamma on epidermal growth factor receptor expression. RESULTS Although interferon gamma elicited 30% to 40% decreases in proliferation, epidermal growth factor receptor expression was strikingly increased in all four ovarian cancer cell lines. Scatchard analysis indicated that this increase occurred primarily at the cell surface, but total cellular receptor levels also were increased. In contrast, interferon gamma treatment of normal ovarian epithelial cells affected neither proliferation nor epidermal growth factor receptor levels. CONCLUSIONS Because the up-regulation of epidermal growth factor receptors by interferon gamma appears to be confined to malignant cells, interferon gamma may facilitate immunotherapy and imaging of ovarian cancers by means of immunoconjugates directed against the epidermal growth factor receptor.

UI MeSH Term Description Entries
D007371 Interferon-gamma The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D009856 Oncogene Proteins, Viral Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities. Viral Oncogene Proteins,Viral Transforming Proteins,v-onc Proteins,Transforming Proteins, Viral,v onc Proteins
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Epidermal growth factor receptor expression in normal and malignant endometrium.
November 1989, American journal of obstetrics and gynecology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Effects of epidermal growth factor on transferrin receptor phosphorylation and surface expression in malignant epithelial cells.
September 1987, Journal of cellular physiology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Expression of epidermal growth factor-related proteins and epidermal growth factor receptor in common epithelial ovarian tumors.
January 1997, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Epidermal growth factor receptor expression in normal ovarian epithelium and ovarian cancer. II. Relationship between receptor expression and response to epidermal growth factor.
March 1991, American journal of obstetrics and gynecology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Effect of epidermal growth factor receptor expression level on survival in patients with epithelial ovarian cancer.
December 2005, Clinical cancer research : an official journal of the American Association for Cancer Research,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells.
January 1990, Endocrinology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Epidermal growth-factor receptor expression in malignant ovary, benign ovarian-tumors and normal ovary - a comparison.
February 1993, International journal of oncology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Interferon-gamma upregulates the c-Met/hepatocyte growth factor receptor expression in alveolar epithelial cells.
October 1999, American journal of respiratory cell and molecular biology,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Modulation by estrogen and growth factors of transforming growth factor-alpha and epidermal growth factor receptor expression in normal and malignant human mammary epithelial cells.
January 1989, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer,
M P Boente, and A Berchuck, and G C Rodriguez, and A Davidoff, and R Whitaker, and F J Xu, and J Marks, and D L Clarke-Pearson, and R C Bast
Expression of the epidermal growth factor receptor family in normal and malignant urothelium.
June 2005, BJU international,
Copied contents to your clipboard!