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In this study we have investigated whether mesothelial cells (MC) and mesothelial extracellular matrix (ECM) are suitable substrates for the adherence of Staphylococci. Mesothelial cells were isolated from the peritoneal dialysis effluent by making use of their lack of Fc-receptors and capacity to attach firmly to plastic. After 10 days post-confluency the MC monolayer was removed with 0.1% Triton-X100 and the presence of an ECM shown by enzyme linked immunosorbent assay (ELISA). The ELISA showed the presence of fibronectin and laminin but not of type IV collagen and vitronectin. Bacterial adherence assays with Staphylococcus aureus (N:3 isolates) adhered well to both ECM (33.4%) and MC monolayers (40.2%; ECM vs. MC monolayers p < 0.03). Staphylococcus aureus adhered significantly better to both ECM (p < 0.05) and MC monolayers (p < 0.05) when compared to plastic. Staphylococcus epidermidis (N:3 isolates) showed similar adherence for plastic (22.1%) and MC monolayers (23.5%); mesothelial ECM was a relatively poor substrate for adherence (6.8%, p < 0.03). In conclusion, results obtained sofar do not indicate an increased risk for adherence of Staphylococci when the mesothelial ECM is exposed.
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
February 1990,
The Journal of infectious diseases,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
September 1986,
Journal of cell science,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
March 2004,
The Journal of antimicrobial chemotherapy,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
December 1985,
Journal of clinical microbiology,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
September 1996,
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
January 1981,
Transactions - American Society for Artificial Internal Organs,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
June 1983,
Cancer research,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
September 1996,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
January 1999,
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis,
M G Betjes, and
C W Tuk, and
D G Struijk, and
R T Krediet, and
L Arisz, and
R H Beelen
January 1997,
Nephron,
