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The effect of diphtheria toxin on certain metabolic processes in growing HeLa cells has been described. It was found that both aerobic respiration and glycolysis are insensitive to toxin action. The incorporation of S(35)-methionine was found to be completely inhibited by toxin prior to the appearance of any visible cell damage. It was also observed that toxin levels above 0.3 Lf per ml. had no further effect on the kinetics of inhibition of methionine uptake. The kinetics of intoxication were studied by adding diphtheria antitoxin to a partially intoxicated culture at various times and measuring the resulting rate of methionine uptake. Low temperatures were found to block intoxication. Using this information, data were obtained suggesting that the initial step in the process of intoxication consists of a rapid adsorption of toxin by the cell. The kinetics of toxin action on protein synthesis were compared with those of cyanide and 2,4-DNP. Diphtheria toxin eliminates completely any methionine uptake remaining after exposure of the cell to either of these respiratory inhibitors. The significance of these results is discussed.
N STRAUSS, and
E D HENDEE
August 1960,
The Journal of experimental medicine,
N STRAUSS, and
E D HENDEE
January 1967,
Postepy higieny i medycyny doswiadczalnej,
N STRAUSS, and
E D HENDEE
January 1976,
Journal of nutritional science and vitaminology,
N STRAUSS, and
E D HENDEE
July 1976,
FEBS letters,
N STRAUSS, and
E D HENDEE
June 1973,
The Journal of biological chemistry,
N STRAUSS, and
E D HENDEE
June 1965,
Biochimica et biophysica acta,
N STRAUSS, and
E D HENDEE
January 1972,
Experientia,
N STRAUSS, and
E D HENDEE
August 1960,
The Journal of experimental medicine,
N STRAUSS, and
E D HENDEE
June 1969,
Journal of bacteriology,
N STRAUSS, and
E D HENDEE
February 1973,
Experimental and molecular pathology,
