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The effect of the somatostatin analog octreotide on cAMP-mediated calcitonin (CT) secretion and cAMP accumulation in C-cells was investigated. Glucagon stimulated cAMP accumulation and CT secretion with a maximal effect at a concentration of 10(-6) M. The cAMP antagonist RpcAMPs blocked the glucagon-induced CT secretion down to control levels. Therefore, no other second messengers seem to be involved in glucagon-stimulated CT secretion. Octreotide in increasing doses (10(-9) to 10(-6) M) inhibited cAMP accumulation and CT secretion with a maximal effect at a concentration of 10(-7) (40% and 29% of control values, respectively). Pretreatment of the cells with 100 ng/mL pertussis toxin for 24 hours abolished the inhibitory effect of octreotide on cAMP accumulation and CT secretion (82% and 58% of control values, respectively). Similar results were obtained under the influence of the phosphodiesterase inhibitor IBMX. Therefore, we conclude that somatostatin modulates adenylate cyclase-coupled CT secretion in C-cells via a pertussis toxin-sensitive G-protein possibly in an autocrine/paracrine way.
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H Scherubl, and
F Raue, and
R Ziegler
August 1992,
Molecular and cellular endocrinology,
A Zink, and
H Scherubl, and
F Raue, and
R Ziegler
October 1993,
FEBS letters,
A Zink, and
H Scherubl, and
F Raue, and
R Ziegler
November 1991,
Endocrinology,
A Zink, and
H Scherubl, and
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R Ziegler
October 1988,
The American journal of physiology,
A Zink, and
H Scherubl, and
F Raue, and
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December 1988,
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A Zink, and
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R Ziegler
January 1994,
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A Zink, and
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R Ziegler
March 1989,
FEBS letters,
A Zink, and
H Scherubl, and
F Raue, and
R Ziegler
June 1992,
British journal of pharmacology,
A Zink, and
H Scherubl, and
F Raue, and
R Ziegler
March 1994,
Biological & pharmaceutical bulletin,
A Zink, and
H Scherubl, and
F Raue, and
R Ziegler
January 1992,
Regulatory peptides,
