BIOMAT Database Logo BIOMAT Database Logo

Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes. 1992

E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Germany.

Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D011831 Radiation Genetics A subdiscipline of genetics that studies RADIATION EFFECTS on the components and processes of biological inheritance. Genetics, Radiation
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D002869 Chromosome Aberrations Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS. Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D002878 Chromosomes, Human, Pair 1 A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification. Chromosome 1
D002881 Chromosomes, Human, Pair 12 A specific pair of GROUP C CHROMOSOMES of the human chromosome classification. Chromosome 12
D002894 Chromosomes, Human, Pair 4 A specific pair of GROUP B CHROMOSOMES of the human chromosome classification. Chromosome 4
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
DNA-proportional distribution of radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization painting of all chromosomes of a human female karyotype.
September 1998, International journal of radiation biology,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Fluorescence in situ hybridization using whole chromosome library probes.
January 1999, Methods in molecular biology (Clifton, N.J.),
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Fluorescence in situ hybridization using whole chromosome library probes.
January 1994, Methods in molecular biology (Clifton, N.J.),
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Automated detection of radiation-induced chromosome aberrations following fluorescence in situ hybridization.
March 1992, Journal of radiation research,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Cervical cytology by means of fluorescence in situ hybridization with a set of chromosome-specific DNA probes.
April 1996, The journal of obstetrics and gynaecology research,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Analysis for DNA-proportional distribution of radiation-induced chromosome aberrations in various triple combinations of human chromosomes using fluorescence in situ hybridization.
June 1994, International journal of radiation biology,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Frequencies of complex chromosome exchange aberrations induced by 238Pu alpha-particles and detected by fluorescence in situ hybridization using single chromosome-specific probes.
April 1995, International journal of radiation biology,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Proficiency testing for laboratories performing fluorescence in situ hybridization with chromosome-specific DNA probes.
December 2002, Archives of pathology & laboratory medicine,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Whole library-amplification and labelling of human chromosome-specific composite probes for fluorescence in situ hybridization (FISH) using PCR.
May 1997, International journal of radiation biology,
E Schmid, and H Zitzelsberger, and H Braselmann, and J W Gray, and M Bauchinger
Incidence of numerical chromosome aberrations in meningioma tumors as revealed by fluorescence in situ hybridization using 10 chromosome-specific probes.
June 2002, Cytometry,
Copied contents to your clipboard!